Immunologic characterization of the membrane-bound collagen in normal human fibroblasts: Identification of a distinct membrane collagen*

Jack R. Lichtenstein, Eugene A. Bauer, Richard Hoyt, H. J. Wedneri

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Collagen, the major extracellular matrix protein, is also a membrane protein. Two types of collagen are detected n the normal human fibroblast membrane in culture, type I collagen and a new immunologically and chemically distinct collagen, type M (membrane) collagen. Antibodies to type M collagen elicited complement-mediated cytotoxicity, which could be blocked by pretreatment of the cells with bacterial collagenase or the antibody with type M collagen. Pretreatment of the cells with other proteolytic enzymes or the antibody with type I collagen or type III collagen had no effect on this complement-mediated cytotexicity. Although type I collagen is the major collagen synthesized by normal human fibroblasts type M collagen may be the major cell membrane collagen and may be a major cell membrane component.

Original languageEnglish
Pages (from-to)145-154
Number of pages10
JournalJournal of Experimental Medicine
Volume144
Issue number1
DOIs
StatePublished - Jul 1 1976

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