TY - JOUR
T1 - Immunologic characterization of the membrane-bound collagen in normal human fibroblasts
T2 - Identification of a distinct membrane collagen*
AU - Lichtenstein, Jack R.
AU - Bauer, Eugene A.
AU - Hoyt, Richard
AU - Wedneri, H. J.
PY - 1976/7/1
Y1 - 1976/7/1
N2 - Collagen, the major extracellular matrix protein, is also a membrane protein. Two types of collagen are detected n the normal human fibroblast membrane in culture, type I collagen and a new immunologically and chemically distinct collagen, type M (membrane) collagen. Antibodies to type M collagen elicited complement-mediated cytotoxicity, which could be blocked by pretreatment of the cells with bacterial collagenase or the antibody with type M collagen. Pretreatment of the cells with other proteolytic enzymes or the antibody with type I collagen or type III collagen had no effect on this complement-mediated cytotexicity. Although type I collagen is the major collagen synthesized by normal human fibroblasts type M collagen may be the major cell membrane collagen and may be a major cell membrane component.
AB - Collagen, the major extracellular matrix protein, is also a membrane protein. Two types of collagen are detected n the normal human fibroblast membrane in culture, type I collagen and a new immunologically and chemically distinct collagen, type M (membrane) collagen. Antibodies to type M collagen elicited complement-mediated cytotoxicity, which could be blocked by pretreatment of the cells with bacterial collagenase or the antibody with type M collagen. Pretreatment of the cells with other proteolytic enzymes or the antibody with type I collagen or type III collagen had no effect on this complement-mediated cytotexicity. Although type I collagen is the major collagen synthesized by normal human fibroblasts type M collagen may be the major cell membrane collagen and may be a major cell membrane component.
UR - http://www.scopus.com/inward/record.url?scp=0017071937&partnerID=8YFLogxK
U2 - 10.1084/jem.144.1.145
DO - 10.1084/jem.144.1.145
M3 - Article
C2 - 932639
AN - SCOPUS:0017071937
SN - 0022-1007
VL - 144
SP - 145
EP - 154
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 1
ER -