TY - JOUR
T1 - Immunohistochemistry of skeletal tissues
AU - Idleburg, Crystal
AU - Delassus, Elizabeth N.
AU - Novack, Deborah V.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Immunohistochemistry (IHC) is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, and then visualizing the bound antibody using a chromogen. Unlike in situ hybridization, which identifies gene transcripts in cells, IHC identifies the products themselves and provides information about their localization within cells (nuclear, cytoplasmic, or membrane) or extracellular matrix. This can be particularly important in the context of bone and cartilage because they contain many cell types as well as matrix components, each with distinct protein expression patterns. As the number of antibodies continues to grow, this technique has become vital for research laboratories studying the skeleton. Here we describe a detailed protocol for IHC analysis of bone and cartilage, addressing specific issues associated with staining of hard and matrix-rich tissues.
AB - Immunohistochemistry (IHC) is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, and then visualizing the bound antibody using a chromogen. Unlike in situ hybridization, which identifies gene transcripts in cells, IHC identifies the products themselves and provides information about their localization within cells (nuclear, cytoplasmic, or membrane) or extracellular matrix. This can be particularly important in the context of bone and cartilage because they contain many cell types as well as matrix components, each with distinct protein expression patterns. As the number of antibodies continues to grow, this technique has become vital for research laboratories studying the skeleton. Here we describe a detailed protocol for IHC analysis of bone and cartilage, addressing specific issues associated with staining of hard and matrix-rich tissues.
KW - Antibodies
KW - Antigen retrieval
KW - Bone
KW - Cartilage
KW - Decalcification
KW - Fixation
KW - Immunohistochemistry
UR - http://www.scopus.com/inward/record.url?scp=84954597036&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-1619-1_8
DO - 10.1007/978-1-4939-1619-1_8
M3 - Article
C2 - 25331045
AN - SCOPUS:84954597036
SN - 1064-3745
VL - 1226
SP - 87
EP - 95
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -