Immunohistochemical Localization of Cyclic GMP in Aggregating Polysphondylium violaceum

PAULINE PAN; H. JAMES WEDNER

doi:10.1111/j.1432-0436.1979.tb01019.x

Immunohistochemical Localization of Cyclic GMP in Aggregating Polysphondylium violaceum

PAULINE PAN
, H. JAMES WEDNER

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Using indirect immunofluorescence technique, it has been possible to localize cyclic GMP in Polysphondylium violaceum cells. The bound cyclic nucleotide is localized throughout the cell during early stages, however, this staining increases and there is marked localization of cyclic GMP in the nuclear areas of the cells when aggregation is in full swing. Over 90% of the cells exhibited intense nuclear staining by 6 h and this decreased to less than 10% by 10 h.

Original language	English
Pages (from-to)	113-118
Number of pages	6
Journal	Differentiation
Volume	14
Issue number	1-3
DOIs	https://doi.org/10.1111/j.1432-0436.1979.tb01019.x
State	Published - 1979

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title = "Immunohistochemical Localization of Cyclic GMP in Aggregating Polysphondylium violaceum",

abstract = "Using indirect immunofluorescence technique, it has been possible to localize cyclic GMP in Polysphondylium violaceum cells. The bound cyclic nucleotide is localized throughout the cell during early stages, however, this staining increases and there is marked localization of cyclic GMP in the nuclear areas of the cells when aggregation is in full swing. Over 90\% of the cells exhibited intense nuclear staining by 6 h and this decreased to less than 10\% by 10 h.",

author = "PAULINE PAN and WEDNER, \{H. JAMES\}",

note = "Funding Information: Acknowledgements: We thank Dr. John T. Bonner for his encouragement and criticism in the preparation of this manuscript and also Dr. Claudette Klein for her helpful comments. This work was supported by research grant BMS 75-09229 from the National Science Foundation and by grant PHS GM-17856 from the National Insti- tute of General Medical Science to Dr. J. T. Bonner and by NIH grants 1 Pol A1 12450 and 1 R01 CA 24430. Dr. Wedner is a recipient of a research career award \#5 KO4 A1 OOO41. We are much obliged to Dr. Peter T. Rowley for the use of his Zeiss fluorescence microscope.",

year = "1979",

doi = "10.1111/j.1432-0436.1979.tb01019.x",

language = "English",

volume = "14",

pages = "113--118",

journal = "Differentiation",

issn = "0301-4681",

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T1 - Immunohistochemical Localization of Cyclic GMP in Aggregating Polysphondylium violaceum

AU - PAN, PAULINE

AU - WEDNER, H. JAMES

N1 - Funding Information: Acknowledgements: We thank Dr. John T. Bonner for his encouragement and criticism in the preparation of this manuscript and also Dr. Claudette Klein for her helpful comments. This work was supported by research grant BMS 75-09229 from the National Science Foundation and by grant PHS GM-17856 from the National Insti- tute of General Medical Science to Dr. J. T. Bonner and by NIH grants 1 Pol A1 12450 and 1 R01 CA 24430. Dr. Wedner is a recipient of a research career award #5 KO4 A1 OOO41. We are much obliged to Dr. Peter T. Rowley for the use of his Zeiss fluorescence microscope.

PY - 1979

Y1 - 1979

N2 - Using indirect immunofluorescence technique, it has been possible to localize cyclic GMP in Polysphondylium violaceum cells. The bound cyclic nucleotide is localized throughout the cell during early stages, however, this staining increases and there is marked localization of cyclic GMP in the nuclear areas of the cells when aggregation is in full swing. Over 90% of the cells exhibited intense nuclear staining by 6 h and this decreased to less than 10% by 10 h.

AB - Using indirect immunofluorescence technique, it has been possible to localize cyclic GMP in Polysphondylium violaceum cells. The bound cyclic nucleotide is localized throughout the cell during early stages, however, this staining increases and there is marked localization of cyclic GMP in the nuclear areas of the cells when aggregation is in full swing. Over 90% of the cells exhibited intense nuclear staining by 6 h and this decreased to less than 10% by 10 h.

UR - https://www.scopus.com/pages/publications/0018295596

U2 - 10.1111/j.1432-0436.1979.tb01019.x

DO - 10.1111/j.1432-0436.1979.tb01019.x

M3 - Article

C2 - 225236

AN - SCOPUS:0018295596

SN - 0301-4681

VL - 14

SP - 113

EP - 118

JO - Differentiation

JF - Differentiation

IS - 1-3

ER -

Immunohistochemical Localization of Cyclic GMP in Aggregating Polysphondylium violaceum

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