Immunohistochemical evaluation of MYC expression in mantle cell lymphoma

Matthew J. Oberley, Saurabh A. Rajguru, Chong Zhang, Kyungmann Kim, Gene R. Shaw, Kreg M. Grindle, Brad S. Kahl, Craig Kanugh, Jennifer Laffin, David T. Yang

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23 Scopus citations

Abstract

Aim: To assess the validity and potential clinical utility of evaluating MYC expression by immunohistochemistry (IHC) in mantle cell lymphoma (MCL). Methods and results: MYC IHC was scored on a tissue microarray containing 62 MCLs and 29 controls by two pathologists. Inter-observer correlation was high (intra-class correlation of 0.98). MYC IHC scores correlated with MYC expression (Spearman's rank correlation 0.69, P < 0.0001) and weakly with Ki67 proliferation index (Spearman's rank correlation 0.30, P = 0.03). Six blastic MCLs did not have higher mean MYC IHC scores or MYC mRNA expression than non-blastic MCLs. None of 57 cases assessed, including all of the blastic cases, showed MYC rearrangement by fluorescence in-situ hybridization. Multivariate analysis with backward selection from potential predictors including age, lactate dehydrogenase, leukocyte count, MIPI score, ECOG performance status, blastic morphology and Ki67 index showed that MYC IHC score is an independent predictor of progression-free survival (hazard ratio 2.34, 95% CI 1.42-3.88, P = 0.0009) and overall survival (hazard ratio 1.90, 95% CI 1.05-3.43, P = 0.034). Conclusions: We show that a new monoclonal anti-MYC antibody can enable accurate and reproducible visual assessment of MYC expression that is independently predictive of clinical outcomes in MCL.

Original languageEnglish
Pages (from-to)499-508
Number of pages10
JournalHistopathology
Volume63
Issue number4
DOIs
StatePublished - Oct 1 2013
Externally publishedYes

Keywords

  • Immunohistochemistry
  • MYC
  • Mantle cell lymphoma
  • Tissue microarray

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  • Cite this

    Oberley, M. J., Rajguru, S. A., Zhang, C., Kim, K., Shaw, G. R., Grindle, K. M., Kahl, B. S., Kanugh, C., Laffin, J., & Yang, D. T. (2013). Immunohistochemical evaluation of MYC expression in mantle cell lymphoma. Histopathology, 63(4), 499-508. https://doi.org/10.1111/his.12207