Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years

Veronika Weyerer, Roland Schneckenpointner, Thomas Filbeck, Maximilian Burger, Ferdinand Hofstaedter, Peter J. Wild, Samson W. Fine, Peter A. Humphrey, Louis P. Dehner, Mahul B. Amin, Josef Rüschoff, Carsten Boltze, Andrea Tannapfel, Ellen Zwarthoff, Antonio Lopez-Beltran, Rodolfo Montironi, Cord Langner, Robert Stoehr, Arndt Hartmann, Johannes Giedl

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Bladder tumours in early-onset patients are rare and seem to exhibit unique clinicopathological features. Only few studies have investigated somatic alterations in this specific age of onset group and evidence is accumulating of a distinct molecular behaviour of early-onset bladder tumours. We collected the largest cohort of early-onset tumours of patients 45 years old or younger and aimed to test genomic alterations typically found in bladder cancer. Tumours of 118 early-onset patients were compared with a consecutive group of 113 cases. Immunohistochemistry of TP53, CK20 and Ki-67 was carried out. Molecular analysis was conducted to test for loss of heterozygosity of chromosome 9 and 17, as well as TP53 and FGFR3 mutations. Fisheŕs exact and chi-squared test were appropriately used. No differences in grade/stage characteristics were observed. Overexpressed TP53 was differentially distributed between the two groups. TP53 nuclear accumulation was significantly more frequent in early-onset papillomas, PUNLMPs and pTa low-grade tumours compared to the consecutive cohort (p=0.005). Moreover, chromosome 9 deletions (29.5% vs. 44.6%) and FGFR3 mutations (34.5% vs. 63.7%) were less often detected in early-onset patients (p=0.05 and p<0.0001). By comparing the largest cohort of early-onset bladder cancer patients with an unselected group, we demonstrated that the typical molecular features are not independent of age at diagnosis. Our study supports the hypothesis of a distinct biological behaviour in early-onset tumours.

Original languageEnglish
Article number482
Pages (from-to)323-331
Number of pages9
JournalJournal of Cancer
Volume8
Issue number3
DOIs
StatePublished - Jan 1 2017

Keywords

  • Bladder cancer
  • Early-onset
  • FGFR3
  • Mutation analysis
  • TP53 positivity

Fingerprint Dive into the research topics of 'Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years'. Together they form a unique fingerprint.

  • Cite this

    Weyerer, V., Schneckenpointner, R., Filbeck, T., Burger, M., Hofstaedter, F., Wild, P. J., Fine, S. W., Humphrey, P. A., Dehner, L. P., Amin, M. B., Rüschoff, J., Boltze, C., Tannapfel, A., Zwarthoff, E., Lopez-Beltran, A., Montironi, R., Langner, C., Stoehr, R., Hartmann, A., & Giedl, J. (2017). Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years. Journal of Cancer, 8(3), 323-331. [482]. https://doi.org/10.7150/jca.17482