Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

A. L. Schwartz; A. Ciechanover; R. A. Brandt; H. J. Geuze

Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

A. L. Schwartz
, A. Ciechanover
, R. A. Brandt
, H. J. Geuze

Research output: Contribution to journal › Article › peer-review

Abstract

Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

Original language	English
Pages (from-to)	2961-2966
Number of pages	6
Journal	The EMBO journal
Volume	7
Issue number	10
State	Published - Oct 1988

Cite this

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title = "Immunoelectron microscopic localization of ubiquitin in hepatoma cells.",

abstract = "Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.",

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year = "1988",

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AU - Schwartz, A. L.

AU - Ciechanover, A.

AU - Brandt, R. A.

AU - Geuze, H. J.

PY - 1988/10

Y1 - 1988/10

N2 - Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

AB - Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

UR - https://www.scopus.com/pages/publications/0024094086

M3 - Article

C2 - 2846272

AN - SCOPUS:0024094086

SN - 0261-4189

VL - 7

SP - 2961

EP - 2966

JO - The EMBO journal

JF - The EMBO journal

IS - 10

ER -

Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

Abstract

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