Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

A. L. Schwartz, A. Ciechanover, R. A. Brandt, H. J. Geuze

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

Original languageEnglish
Pages (from-to)2961-2966
Number of pages6
JournalThe EMBO journal
Issue number10
StatePublished - Oct 1988


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