Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

A. L. Schwartz; A. Ciechanover; R. A. Brandt; H. J. Geuze

Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

A. L. Schwartz, A. Ciechanover, R. A. Brandt, H. J. Geuze

Research output: Contribution to journal › Article › peer-review

Abstract

Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

Original language	English
Pages (from-to)	2961-2966
Number of pages	6
Journal	The EMBO journal
Volume	7
Issue number	10
State	Published - Oct 1988

Cite this

@article{87cb76a46a2549dd9cbc66a45c0ab64c,

title = "Immunoelectron microscopic localization of ubiquitin in hepatoma cells.",

abstract = "Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.",

author = "Schwartz, {A. L.} and A. Ciechanover and Brandt, {R. A.} and Geuze, {H. J.}",

year = "1988",

month = oct,

language = "English",

volume = "7",

pages = "2961--2966",

journal = "The EMBO journal",

issn = "0261-4189",

number = "10",

}

TY - JOUR

T1 - Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

AU - Schwartz, A. L.

AU - Ciechanover, A.

AU - Brandt, R. A.

AU - Geuze, H. J.

PY - 1988/10

Y1 - 1988/10

N2 - Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

AB - Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

UR - http://www.scopus.com/inward/record.url?scp=0024094086&partnerID=8YFLogxK

M3 - Article

C2 - 2846272

AN - SCOPUS:0024094086

SN - 0261-4189

VL - 7

SP - 2961

EP - 2966

JO - The EMBO journal

JF - The EMBO journal

IS - 10

ER -

Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

Abstract

Other files and links

Fingerprint

Cite this