Immunoelectron microscopic localization of acidic intracellular compartments in hepatoma cells.

A. L. Schwartz, G. J. Strous, J. W. Slot, H. J. Geuze

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Using protein A-colloidal gold immunoelectron microscopy and monospecific antibodies to the weak base primaquine, we have delineated acidic intracellular compartments in the human hepatoma cell line, HepG2. Primaquine specifically accumulated within endocytotic compartments (including CURL vesicles, multivesicular bodies and lysosomes). In addition, the Golgi cisternae were positive. However, the CURL tubules, which contain recycling asialoglycoprotein receptor, did not accumulate primaquine. Thus, there may be a gradient of acidification within the endocytotic pathway.

Original languageEnglish
Pages (from-to)899-904
Number of pages6
JournalThe EMBO journal
Volume4
Issue number4
DOIs
StatePublished - Apr 1985

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