@inbook{27c764abf74b4d4f9c4f684e3a4835d8,
title = "Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells",
abstract = "Immunoaffinity purification allows for the purification of epitope-tagged proteins and their associated multisubunit complexes from mammalian cells. Subsequent identification of the proteins by proteomic analysis enables unbiased biochemical characterization of their associated partners, potentially revealing the physiological or functional context of any given protein. Here, we use immunoaffinity isolation of the Activating Signal Co-integrator Complex (ASCC) from human cells as an example, demonstrating the utility of the approach in revealing protein complexes involved in genotoxic stress responses.",
keywords = "ASCC, Mass spectrometry, Protein purification",
author = "Townley, {Brittany A.} and Soll, {Jennifer M.} and Nima Mosammaparast",
note = "Funding Information: Work in our laboratory is supported by the NIH (R01 CA193318, R01 CA227001, and P01 CA092584), the American Cancer Society research scholar program (RSG-18-156-01-DMC), and the Alvin J. Siteman Cancer Center Investment Program, which is supported by the Foundation for Barnes-Jewish Hospital Cancer Frontier Fund and the National Cancer Institute, Cancer Support Grant P30 CA091842. B.A.T. is supported by the NCI Ruth L. Kirschstein National Research Service Award (NCI F31CA254143). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2063-2_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "29--41",
booktitle = "Methods in Molecular Biology",
}