Immune system dysfunction and autoimmune disease in mice lacking Emk (Par-1) protein kinase

J. B. Hurov, T. S. Stappenbeck, C. M. Zmasek, L. S. White, S. H. Ranganath, J. H. Russell, A. C. Chan, K. M. Murphy, H. Piwnica-Worms

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

Emk is a serine/threonine protein kinase implicated in regulating polarity, cell cycle progression, and microtubule dynamics. To delineate the role of Emk in development and adult tissues, mice lacking Emk were generated by targeted gene disruption. Emk-/- mice displayed growth retardation and immune cell dysfunction. Although B- and T-cell development were normal, CD4+T cells lacking Emk exhibited a marked upregulation of the memory marker CD44/pgp-1 and produced more gamma interferon and interleukin-4 on stimulation through the T-cell receptor in vitro. In addition, B-cell responses to T-cell-dependent and -independent antigen challenge were altered in vivo. As Emk-/- animals aged, they developed splenomegaly, lymphadenopathy, membranoproliferative glomerulonephritis, and lymphocytic infiltrates in the lungs, parotid glands and kidneys. Taken together, these results demonstrate that the Emk protein kinase is essential for maintaining immune system homeostasis and that loss of Emk may contribute to autoimmune disease in mammals.

Original languageEnglish
Pages (from-to)3206-3219
Number of pages14
JournalMolecular and cellular biology
Volume21
Issue number9
DOIs
StatePublished - 2001

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