Immune complexes inhibit antimicrobial responses through interleukin-10 production. Effects in severe combined immunodeficient mice during Listeria infection

C. S. Tripp, K. P. Beckerman, E. R. Unanue

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

The presence of soluble antigen-antibody complexes renders mice highly susceptible to infection with the intracellular pathogen Listeria monocytogenes. In this report we show that this inhibition is manifest at the level of the innate immune response and is mediated by IL-10. Like immunocompetent mice, mice with the severe combined immunodeficient mutation (SCID) injected with immune complexes died from a sublethal dose of L. monocytogenes. These mice were protected if pretreated with neutralizing antibodies to IL-10. In vitro, immune complexes stimulated IL-10 production by SCID splenocytes and splenic macrophages. Likewise, immune complexes inhibited TNF and IFN-γ production by SCID splenocytes cultured with heat- killed-L. monocytogenes. This inhibition was reversed by neutralization of IL-10 but not IL-4 or TGF-β. Immune complexes and rIL-10 inhibited cytokine production by SCID splenocytes if added before or simultaneously with heat- killed-L. monocytogenes. These data support a model in which immune complexes modulate host defense and the immune response by stimulating the production of IL-10 from macrophages.

Original languageEnglish
Pages (from-to)1628-1634
Number of pages7
JournalJournal of Clinical Investigation
Volume95
Issue number4
DOIs
StatePublished - Jan 1 1995

Keywords

  • cytokines
  • innate immunity
  • macrophages
  • microbial infection
  • natural killer cell

Fingerprint Dive into the research topics of 'Immune complexes inhibit antimicrobial responses through interleukin-10 production. Effects in severe combined immunodeficient mice during Listeria infection'. Together they form a unique fingerprint.

  • Cite this