TY - JOUR
T1 - Immobilization of polysaccharides on a fluorinated silicon surface
AU - Wang, Anfeng
AU - Cao, Ting
AU - Tang, Haiying
AU - Liang, Xuemei
AU - Black, Carolyn
AU - Salley, Steven O.
AU - McAllister, James P.
AU - Auner, Gregory W.
AU - Ng, K. Y.Simon
N1 - Funding Information:
Financial support of this research from the Michigan Life Science Corridor (MLSC) is gratefully acknowledged. We would also like to thank Prof. Guangzhao Mao (Department of Chemical Engineering and Materials Science, Wayne State University) for the use of AFM and contact angle goniometer.
PY - 2006/1/15
Y1 - 2006/1/15
N2 - A self-assembled monolayer (SAM) of fluoroalkyl silane (FAS) was deposited on a silicon surface by chemical vapor deposition (CVD) at room temperature under 1.01 × 105 Pa nitrogen. Using this new approach, the quality and reproducibility of the SAM are better than those prepared either in solution or by vapor phase deposition, and the deposition process is simpler. In this modified CVD process, the silane monomers, instead of the oligomeric species, are the primary reactants. Full coverage of the silicon surface by FAS molecules was achieved within 5 min. Heparin and hyaluronan, two naturally occurring biocompatible polysaccharides, were successfully covalently attached on the FAS SAM/Si surface by photo-immobilization. Atomic force microscopy (AFM) revealed the morphologic changes after the immobilization of heparin and hyaluronan, and X-ray photoelectron spectroscopy (XPS) confirmed the change in chemical compositions. Such combination of coatings is expected to enhance the stability and biocompatibility of the base material.
AB - A self-assembled monolayer (SAM) of fluoroalkyl silane (FAS) was deposited on a silicon surface by chemical vapor deposition (CVD) at room temperature under 1.01 × 105 Pa nitrogen. Using this new approach, the quality and reproducibility of the SAM are better than those prepared either in solution or by vapor phase deposition, and the deposition process is simpler. In this modified CVD process, the silane monomers, instead of the oligomeric species, are the primary reactants. Full coverage of the silicon surface by FAS molecules was achieved within 5 min. Heparin and hyaluronan, two naturally occurring biocompatible polysaccharides, were successfully covalently attached on the FAS SAM/Si surface by photo-immobilization. Atomic force microscopy (AFM) revealed the morphologic changes after the immobilization of heparin and hyaluronan, and X-ray photoelectron spectroscopy (XPS) confirmed the change in chemical compositions. Such combination of coatings is expected to enhance the stability and biocompatibility of the base material.
KW - Chemical vapor deposition
KW - Fluoroalkyl silane
KW - Heparin
KW - Hyaluronan
KW - Photo-immobilization
KW - Silicon
UR - http://www.scopus.com/inward/record.url?scp=30944461570&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2005.11.016
DO - 10.1016/j.colsurfb.2005.11.016
M3 - Article
C2 - 16387479
AN - SCOPUS:30944461570
SN - 0927-7765
VL - 47
SP - 57
EP - 63
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 1
ER -