Immature CD4+CD8+ thymocytes form a multifocal immunological synapse with sustained tyrosine phosphorylation

Eric Hailman, W. Richard Burack, Andrey S. Shaw, Michael L. Dustin, Paul M. Allen

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

The immunological synapse formed during mature T cell activation consists of a central cluster of TCR and MHC molecules surrounded by a ring of LFA-1 and ICAM-1. We examined synapse formation in thymocytes undergoing activation in a lipid bilayer system by following the movement of fluorescent MHC and ICAM-1 molecules. Immature CD4+CD8+ thymocytes formed a decentralized synapse with multiple foci of MHC accumulation corresponding to areas of exclusion of ICAM-1. The MHC clusters and ICAM-1 holes were mobile and transient and correlated with active and sustained signaling, as shown by staining with antibodies against phosphotyrosine and activated Lck. Our findings show that signaling in immature thymocytes can result from a novel, multifocal pattern of receptor accumulation.

Original languageEnglish
Pages (from-to)839-848
Number of pages10
JournalImmunity
Volume16
Issue number6
DOIs
StatePublished - 2002

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