Imaging techniques in retinal research

Josh Morgan, Rachel Huckfeldt, Rachel O.L. Wong

Research output: Contribution to journalReview articlepeer-review

20 Scopus citations

Abstract

In recent years, retinal research has benefited from major advances in optical imaging approaches. Investigations of the structural and functional organization of the vertebrate retina using live preparations have been facilitated by improvements in cell labeling methods, and by microscopy techniques that permit high-resolution of cells in vitro and in vivo. In particular, the generation of transgenic animals with fluorescently labeled retinal cells has permitted real-time visualization of cell generation, migration, differentiation and growth in the developing retina. Neuronal activity can also be examined by optical imaging using activity reporters directed to specific retinal cell types. Optical techniques such as multiphoton microscopy and total internal reflection fluorescence microscopy (TIRFM) have helped unravel the physiological properties and function of retinal cells. Here, we focus on the latest cell labeling methods that have proven highly useful in many aspects of retinal research. We also highlight several examples of how newly developed imaging technology itself has facilitated investigations that have advanced our understanding of retinal circuits and their development.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalExperimental eye research
Volume80
Issue number3
DOIs
StatePublished - Mar 2005

Keywords

  • Cell labeling methods
  • Fluorescent proteins
  • Live cell imaging
  • Optical imaging
  • Retinal development
  • Retinal function

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