TY - JOUR
T1 - Imaging lung inflammation in a murine model of Pseudomonas infection
T2 - A positron emission tomography study
AU - Schuster, Daniel P.
AU - Kozlowski, James
AU - Hogue, Lisa
AU - Ferkol, Thomas W.
N1 - Funding Information:
Received 10 June 2002; accepted 8 August 2002. This work was supported by NIH HL32815, HL13851, and HL64044, and the Cystic Fibrosis Foundation. The authors gratefully acknowledge the expert technical assistance provided by the support staff of the microPET facility in the Division of Radiation Sciences. Address correspondence to Daniel Schuster MD, University Box 8225, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA. E-mail: [email protected]
PY - 2003/1
Y1 - 2003/1
N2 - We tested the hypothesis that the uptake of [18F]fluorodeoxyglucose (FDG), as measured by positron emission tomography (PET) imaging, would correlate with inflammation caused by increasing doses of instilled Pseudomonas aeruginosa (PA) into the lungs of mice. PA-laden agarose beads were instilled via the trachea into 1 lung of each mouse (dose range 0.5-15 × 104 CFU) and imaging was performed 3 days later (at the peak of the inflammatory response). Lung uptake of [18F]FDG correlated significantly with the dose of bacteria instilled in mice infected with the M57-15 strain of PA (n= 18) (r2= .62), but not in mice infected with the PA01 strain (n= 20). The overall lung uptake of [18F]FDG was higher in mice infected with the M57-15 strain than in those infected with the PA01 strain (P <.05). Total white blood cell concentrations in bronchoalveolar lavage were also higher in the M57-15-infected mice. We conclude that PET imaging can detect and quantify differences in host inflammatory response to 2 different strains of PA. The combination of PET imaging with routine models should be a useful new tool to study neutrophil trafficking and kinetics in lung inflammation.
AB - We tested the hypothesis that the uptake of [18F]fluorodeoxyglucose (FDG), as measured by positron emission tomography (PET) imaging, would correlate with inflammation caused by increasing doses of instilled Pseudomonas aeruginosa (PA) into the lungs of mice. PA-laden agarose beads were instilled via the trachea into 1 lung of each mouse (dose range 0.5-15 × 104 CFU) and imaging was performed 3 days later (at the peak of the inflammatory response). Lung uptake of [18F]FDG correlated significantly with the dose of bacteria instilled in mice infected with the M57-15 strain of PA (n= 18) (r2= .62), but not in mice infected with the PA01 strain (n= 20). The overall lung uptake of [18F]FDG was higher in mice infected with the M57-15 strain than in those infected with the PA01 strain (P <.05). Total white blood cell concentrations in bronchoalveolar lavage were also higher in the M57-15-infected mice. We conclude that PET imaging can detect and quantify differences in host inflammatory response to 2 different strains of PA. The combination of PET imaging with routine models should be a useful new tool to study neutrophil trafficking and kinetics in lung inflammation.
KW - Emission-computed tomography
KW - Pneumonia
KW - Pseudomonas aeruginosa
KW - [F]fludeoxyglucose
UR - http://www.scopus.com/inward/record.url?scp=0037216781&partnerID=8YFLogxK
U2 - 10.1080/01902140303760
DO - 10.1080/01902140303760
M3 - Article
C2 - 12652815
AN - SCOPUS:0037216781
SN - 0190-2148
VL - 29
SP - 45
EP - 57
JO - Experimental Lung Research
JF - Experimental Lung Research
IS - 1
ER -