Abstract
The disease-causing genetic information might reside in the genomic DNA or RNA, or in a virus, bacterium, or other pathogen, or from alterations in the methylation pattern of DNA, which in turn alters RNA expression levels. A much more attractive and universal intracellular target from a design point of view would be to directly target genetic information associated with a disease state because information in the form of DNA or RNA can in principle be easily recognized by Watson-Crick base pairing with an antisense oligonucleotide. There are two general classes of reporter systems that can be used such as always ON and turn-on reporter systems. Most of the literature concerning genetically targeted nanoparticle (NP), deals with siRNA and gene therapy, and very little deals with antisense imaging.
Original language | English |
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Title of host publication | Nanotechnology for Biomedical Imaging and Diagnostics |
Subtitle of host publication | From Nanoparticle Design to Clinical Applications |
Publisher | wiley |
Pages | 373-400 |
Number of pages | 28 |
ISBN (Electronic) | 9781118873151 |
ISBN (Print) | 9781118121184 |
DOIs | |
State | Published - Jan 30 2015 |
Keywords
- Antisense imaging
- Nanoparticle
- Nucleic acids
- Reporter systems