Imaging ATUM ultrathin section libraries with WaferMapper: A multi-scale approach to EM reconstruction of neural circuits

Kenneth J. Hayworth, Josh L. Morgan, Richard Schalek, Daniel R. Berger, David G.C. Hildebrand, Jeff W. Lichtman

Research output: Contribution to journalArticle

108 Scopus citations

Abstract

The automated tape-collecting ultramicrotome (ATUM) makes it possible to collect large numbers of ultrathin sections quickly-the equivalent of a petabyte of high resolution images each day. However, even high throughput image acquisition strategies generate images far more slowly (at present ~1 terabyte per day). We therefore developed WaferMapper, a software package that takes a multi-resolution approach to mapping and imaging select regions within a library of ultrathin sections. This automated method selects and directs imaging of corresponding regions within each section of an ultrathin section library (UTSL) that may contain many thousands of sections. Using WaferMapper, it is possible to map thousands of tissue sections at low resolution and target multiple points of interest for high resolution imaging based on anatomical landmarks. The program can also be used to expand previously imaged regions, acquire data under different imaging conditions, or re-image after additional tissue treatments.

Original languageEnglish
Article number68
JournalFrontiers in Neural Circuits
Volume8
Issue numberJUNE
DOIs
StatePublished - Jun 27 2014

Keywords

  • ATUM
  • Connectomics
  • Imaging software
  • Scanning electron microscopy
  • Serial-section electron microscopy
  • Tape collection
  • Ultramicrotome
  • Volume EM

Fingerprint Dive into the research topics of 'Imaging ATUM ultrathin section libraries with WaferMapper: A multi-scale approach to EM reconstruction of neural circuits'. Together they form a unique fingerprint.

  • Cite this