The human kinome, with more than 500 proteins, is crucial for cell signaling and disease. Yet, about one-third of kinases lack in-depth study. The Data and Resource Generating Center for Understudied Kinases has developed multiple resources to address this challenge including creation of a heavy amino acid peptide library for parallel reaction monitoring and quantitation of protein kinase expression, use of understudied kinases tagged with a miniTurbo-biotin ligase to determine interaction networks by proximity-dependent protein biotinylation, NanoBRET probe development for screening chemical tool target specificity in live cells, characterization of small molecule chemical tools inhibiting understudied kinases, and computational tools for defining kinome architecture. These resources are available through the Dark Kinase Knowledgebase, supporting further research into these understudied protein kinases.

Original languageEnglish
Article number103881
JournalDrug Discovery Today
Issue number3
StatePublished - Mar 2024


  • NanoBRET
  • SureQuant/PRM
  • chemical tools
  • dark kinase knowledgebase
  • human kinome
  • illuminating the druggable genome
  • miniTurbo
  • understudied kinases


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