TY - JOUR
T1 - IL-1β stabilizes COX II mRNA in renal mesangial cells
T2 - Role of 3'- untranslated region
AU - Srivastava, S. K.
AU - Tetsuka, T.
AU - Daphna-Iken, D.
AU - Morrison, A. R.
PY - 1994
Y1 - 1994
N2 - We stimulated rat mesangial cells for different time intervals with interleukin-1β (IL-1β) and phorbol 12-myristate 13-acetate, prepared cytoplasmic extracts, and examined these extracts for the presence of RNA binding proteins by gel mobility shift assays. Here we report that the 3'- untranslated region (3'-UNTR) of the prostaglandin endoperoxide synthase II (COX II) gene is responsible for posttranscriptional regulation of the response to IL-1β. Two cytosolic transacting factors of 65 and 45 kDa, respectively, have been detected that bind to the 3'-UNTR. Competition with excess RNA and acid phosphatase treatment of the cytoplasmic extract suggest the binding is specific and that phosphorylation is required for these rapid binding events. These experiments suggest that IL-1β induces the phosphorylation of cytosolic factors, which bind to the 3'-UNTR of COX II mRNA, and stabilizes the message.
AB - We stimulated rat mesangial cells for different time intervals with interleukin-1β (IL-1β) and phorbol 12-myristate 13-acetate, prepared cytoplasmic extracts, and examined these extracts for the presence of RNA binding proteins by gel mobility shift assays. Here we report that the 3'- untranslated region (3'-UNTR) of the prostaglandin endoperoxide synthase II (COX II) gene is responsible for posttranscriptional regulation of the response to IL-1β. Two cytosolic transacting factors of 65 and 45 kDa, respectively, have been detected that bind to the 3'-UNTR. Competition with excess RNA and acid phosphatase treatment of the cytoplasmic extract suggest the binding is specific and that phosphorylation is required for these rapid binding events. These experiments suggest that IL-1β induces the phosphorylation of cytosolic factors, which bind to the 3'-UNTR of COX II mRNA, and stabilizes the message.
KW - cyclooxygenase
KW - interleukin-1β
KW - phosphorylation
KW - ribonucleic acid-protein interaction
UR - http://www.scopus.com/inward/record.url?scp=0027967245&partnerID=8YFLogxK
M3 - Article
C2 - 8092265
AN - SCOPUS:0027967245
SN - 0363-6127
VL - 267
SP - F504-F508
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
IS - 3 36-3
ER -