IFT proteins accumulate during cell division and localize to the cleavage furrow in chlamydomonas

Christopher R. Wood, Zhaohui Wang, Dennis Diener, James Matt Zones, Joel Rosenbaum, James G. Umen

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Intraflagellar transport (IFT) proteins are well established as conserved mediators of flagellum/cilium assembly and disassembly. However, data has begun to accumulate in support of IFT protein involvement in other processes elsewhere in the cell. Here, we used synchronous cultures of Chlamydomonas to investigate the temporal patterns of accumulation and localization of IFT proteins during the cell cycle. Their mRNAs showed periodic expression that peaked during S and M phase (S/M). Unlike most proteins that are synthesized continuously during G1 phase, IFT27 and IFT46 levels were found to increase only during S/M phase. During cell division, IFT27, IFT46, IFT72, and IFT139 re-localized from the flagella and basal bodies to the cleavage furrow. IFT27 was further shown to be associated with membrane vesicles in this region. This localization pattern suggests a role for IFT in cell division.

Original languageEnglish
Article numbere30729
JournalPloS one
Volume7
Issue number2
DOIs
StatePublished - Feb 6 2012

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