TY - JOUR
T1 - IFT proteins accumulate during cell division and localize to the cleavage furrow in chlamydomonas
AU - Wood, Christopher R.
AU - Wang, Zhaohui
AU - Diener, Dennis
AU - Zones, James Matt
AU - Rosenbaum, Joel
AU - Umen, James G.
PY - 2012/2/6
Y1 - 2012/2/6
N2 - Intraflagellar transport (IFT) proteins are well established as conserved mediators of flagellum/cilium assembly and disassembly. However, data has begun to accumulate in support of IFT protein involvement in other processes elsewhere in the cell. Here, we used synchronous cultures of Chlamydomonas to investigate the temporal patterns of accumulation and localization of IFT proteins during the cell cycle. Their mRNAs showed periodic expression that peaked during S and M phase (S/M). Unlike most proteins that are synthesized continuously during G1 phase, IFT27 and IFT46 levels were found to increase only during S/M phase. During cell division, IFT27, IFT46, IFT72, and IFT139 re-localized from the flagella and basal bodies to the cleavage furrow. IFT27 was further shown to be associated with membrane vesicles in this region. This localization pattern suggests a role for IFT in cell division.
AB - Intraflagellar transport (IFT) proteins are well established as conserved mediators of flagellum/cilium assembly and disassembly. However, data has begun to accumulate in support of IFT protein involvement in other processes elsewhere in the cell. Here, we used synchronous cultures of Chlamydomonas to investigate the temporal patterns of accumulation and localization of IFT proteins during the cell cycle. Their mRNAs showed periodic expression that peaked during S and M phase (S/M). Unlike most proteins that are synthesized continuously during G1 phase, IFT27 and IFT46 levels were found to increase only during S/M phase. During cell division, IFT27, IFT46, IFT72, and IFT139 re-localized from the flagella and basal bodies to the cleavage furrow. IFT27 was further shown to be associated with membrane vesicles in this region. This localization pattern suggests a role for IFT in cell division.
UR - http://www.scopus.com/inward/record.url?scp=84856630786&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0030729
DO - 10.1371/journal.pone.0030729
M3 - Article
C2 - 22328921
AN - SCOPUS:84856630786
SN - 1932-6203
VL - 7
JO - PLoS ONE
JF - PLoS ONE
IS - 2
M1 - e30729
ER -