Identifying protein-binding sites from unaligned DNA fragments

G. D. Stormo, G. W. Hartzell

Research output: Contribution to journalArticlepeer-review

349 Scopus citations

Abstract

The ability to determine important features within DNA sequences from the sequences alone is becoming essential as large-scale sequencing projects are being undertaken. We present a method that can be applied to the problem of identifying the recognition pattern for a DNA-binding protein given only a collection of sequenced DNA fragments, each known to contain somewhere within it a binding site for that protein. Information about the position or orientation of the binding sites within those fragments is not needed. The method compares the 'information content' of a large number of possible binding site alignments to arrive at a matrix representation of the binding site pattern. The specificity of the protein is represented as a matrix, rather than a consensus sequence, allowing patterns that are typical of regulatory protein-binding sites to be identified. The reliability of the method improves as the number of sequences increases, but the time required increases only linearly with the number of sequences. An example, using known cAMP receptor protein binding sites, illustrates the method.

Original languageEnglish
Pages (from-to)1183-1187
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number4
DOIs
StatePublished - 1989

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