TY - JOUR
T1 - Identification of tyrosine phosphorylated adhesion proteins in human cancer cells
AU - Kinch, Michael S.
AU - Kilpatrick, Katherine E.
AU - Zhong, Cuiling
PY - 1998
Y1 - 1998
N2 - Tyrosine phosphorylation is a form of signal transduction that regulates cell growth, differentiation, migration, and survival. This knowledge has promoted much interest in the role of tyrosine kinases and phosphatases in regulating cell behavior during development and tumorigenesis. However, it is generally less well appreciated that tyrosine phosphorylated proteins are enriched within sites of cell adhesion, particularly in transformed cells. To identify these, we developed a panel of monoclonal antibodies specific for tyrosine phosphorylated proteins in breast cancer cells, using extensive modifications of existing technologies for immunization, somatic fusion, and antibody screening. Mice were immunized with a complex mixture of phosphotyrosine containing proteins using the newly developed RIMMS method. By increasing the sensitivity of antigen recognition, we isolated reagents specific for a wide diversity of tyrosine phosphorylated adhesion proteins in breast cancer cells.
AB - Tyrosine phosphorylation is a form of signal transduction that regulates cell growth, differentiation, migration, and survival. This knowledge has promoted much interest in the role of tyrosine kinases and phosphatases in regulating cell behavior during development and tumorigenesis. However, it is generally less well appreciated that tyrosine phosphorylated proteins are enriched within sites of cell adhesion, particularly in transformed cells. To identify these, we developed a panel of monoclonal antibodies specific for tyrosine phosphorylated proteins in breast cancer cells, using extensive modifications of existing technologies for immunization, somatic fusion, and antibody screening. Mice were immunized with a complex mixture of phosphotyrosine containing proteins using the newly developed RIMMS method. By increasing the sensitivity of antigen recognition, we isolated reagents specific for a wide diversity of tyrosine phosphorylated adhesion proteins in breast cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=0031826196&partnerID=8YFLogxK
U2 - 10.1089/hyb.1998.17.227
DO - 10.1089/hyb.1998.17.227
M3 - Article
C2 - 9708824
AN - SCOPUS:0031826196
SN - 0272-457X
VL - 17
SP - 227
EP - 235
JO - Hybridoma
JF - Hybridoma
IS - 3
ER -