TY - JOUR
T1 - Identification of the fifth subunit os Saccharomyces cerevisiae replication factor C
AU - Gary, Sonja L.
AU - Burgers, Peter M.J.
N1 - Funding Information:
We thank Kim Impellizzeri, Bonnie Yoder and Jonathon Wong for technical support, John Majors and Chris Hardy for critical discussions and Connie Holm for antisera against Rfclp. This work was supported in part by grant GM32431 from the National Institutes of Health.
PY - 1995
Y1 - 1995
N2 - Yeast replication factor C (RF-C) is a muttipolypeptide complex required for chromosomal DNA replication. Previously this complex was known to consist of at least four subunits. We here report the Identification of a fifth RF-C subunit from Saccharomyces cerevisiae, encoded by the RFC5 (YBR0810) gene. This subunit exhibits highest homology to the 38 kDa subunit (38%) of human RF-C (activator 1). Like the other four RFC genes, the RFC5 gene Is essential for yeast viability, indicating an essential function for each subunit. RFC5 mRNA is expressed at steady-state levels throughout the mitotic cell cycle. Upon overexpression in Escherichla coli Rfc5p has an apparent molecular mass of 41 kDa. Overproduction of RF-C activity in yeast is dependent on overexpression of the RFC5 gene together with overexpression of the RFC1-4 genes, indicating that the RFC5 gene product forms an integral subunit of this replication factor.
AB - Yeast replication factor C (RF-C) is a muttipolypeptide complex required for chromosomal DNA replication. Previously this complex was known to consist of at least four subunits. We here report the Identification of a fifth RF-C subunit from Saccharomyces cerevisiae, encoded by the RFC5 (YBR0810) gene. This subunit exhibits highest homology to the 38 kDa subunit (38%) of human RF-C (activator 1). Like the other four RFC genes, the RFC5 gene Is essential for yeast viability, indicating an essential function for each subunit. RFC5 mRNA is expressed at steady-state levels throughout the mitotic cell cycle. Upon overexpression in Escherichla coli Rfc5p has an apparent molecular mass of 41 kDa. Overproduction of RF-C activity in yeast is dependent on overexpression of the RFC5 gene together with overexpression of the RFC1-4 genes, indicating that the RFC5 gene product forms an integral subunit of this replication factor.
UR - http://www.scopus.com/inward/record.url?scp=0029597423&partnerID=8YFLogxK
U2 - 10.1093/nar/23.24.4986
DO - 10.1093/nar/23.24.4986
M3 - Article
C2 - 8559655
AN - SCOPUS:0029597423
SN - 0305-1048
VL - 23
SP - 4986
EP - 4991
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 24
ER -