Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily

Alison H. Banham, Marco Colonna, Marina Cella, Kingsley J. Micklem, Karen Pulford, Antony C. Willis, David Y. Mason

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The CD85 molecule was originally defined at the Fifth Workshop on Leucocyte Antigens in 1993 by two monoclonal antibodies, VMP55 and GHI/75. This cell-surface glycoprotein is expressed on B cells, monocytes, and subpopulations of T and natural killer (NK) cells, and particularly high levels are expressed by normal and neoplastic plasma cells and by hairy cell leukemia B cells. We affinity purified the CD85 antigen and obtained tryptic peptide sequence which indicated that this molecule might be ILT2, a recently described inhibitory major histocompatibility complex class I receptor of the immunoglobulin superfamily. This was confirmed by showing that both of the original anti-CD85 mAbs stained ILT2 transfectants. The cell signaling role demonstrated for ILT2 is consistent with the previously reported involvement of CD85 in T cell activation.

Original languageEnglish
Pages (from-to)841-845
Number of pages5
JournalJournal of Leukocyte Biology
Volume65
Issue number6
DOIs
StatePublished - 1999

Keywords

  • B lymphocytes
  • Monoclonal antibodies
  • Signal transduction

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