Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization

Zhuoming Liu; Laura A. VanBlargan; Louis Marie Bloyet; Paul W. Rothlauf; Rita E. Chen; Spencer Stumpf; Haiyan Zhao; John M. Errico; Elitza S. Theel; Mariel J. Liebeskind; Brynn Alford; William J. Buchser; Ali H. Ellebedy; Daved H. Fremont; Michael S. Diamond; Sean P.J. Whelan

doi:10.1016/j.chom.2021.01.014

Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization

Zhuoming Liu
, Laura A. VanBlargan
, Louis Marie Bloyet
, Paul W. Rothlauf
, Rita E. Chen
, Spencer Stumpf
, Haiyan Zhao
, John M. Errico
, Elitza S. Theel
, Mariel J. Liebeskind
, Brynn Alford
, William J. Buchser
, Ali H. Ellebedy
, Daved H. Fremont
, Michael S. Diamond
, Sean P.J. Whelan

Research output: Contribution to journal › Article › peer-review

536 Scopus citations

Abstract

Liu et al. isolated 50 escape mutants to 19 monoclonal antibodies targeting the SARS-CoV-2 spike using a VSV-SARS2 chimera. Some mutants (S477N) resist neutralization by multiple mAbs, and others (E484K) are less sensitive to neutralization by convalescent sera. Several mutants exist in clinical SARS2-CoV-2 isolates and thus warrant further study.

Original language	English
Pages (from-to)	477-488.e4
Journal	Cell Host and Microbe
Volume	29
Issue number	3
DOIs	https://doi.org/10.1016/j.chom.2021.01.014
State	Published - Mar 10 2021

Keywords

ACE2 receptor decoys
COVID-19 vaccines
SARS-CoV-2 escape mutants
convalescent sera
coronaviruses
monoclonal antibodies
receptor-binding domain

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10.1016/j.chom.2021.01.014

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Liu, Z., VanBlargan, L. A., Bloyet, L. M., Rothlauf, P. W., Chen, R. E., Stumpf, S., Zhao, H., Errico, J. M., Theel, E. S., Liebeskind, M. J., Alford, B., Buchser, W. J., Ellebedy, A. H., Fremont, D. H., Diamond, M. S., & Whelan, S. P. J. (2021). Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization. Cell Host and Microbe, 29(3), 477-488.e4. https://doi.org/10.1016/j.chom.2021.01.014

@article{b71d05d9dfd545e98272c4c4d84366bd,

title = "Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization",

abstract = "Liu et al. isolated 50 escape mutants to 19 monoclonal antibodies targeting the SARS-CoV-2 spike using a VSV-SARS2 chimera. Some mutants (S477N) resist neutralization by multiple mAbs, and others (E484K) are less sensitive to neutralization by convalescent sera. Several mutants exist in clinical SARS2-CoV-2 isolates and thus warrant further study.",

keywords = "ACE2 receptor decoys, COVID-19 vaccines, SARS-CoV-2 escape mutants, convalescent sera, coronaviruses, monoclonal antibodies, receptor-binding domain",

author = "Zhuoming Liu and VanBlargan, \{Laura A.\} and Bloyet, \{Louis Marie\} and Rothlauf, \{Paul W.\} and Chen, \{Rita E.\} and Spencer Stumpf and Haiyan Zhao and Errico, \{John M.\} and Theel, \{Elitza S.\} and Liebeskind, \{Mariel J.\} and Brynn Alford and Buchser, \{William J.\} and Ellebedy, \{Ali H.\} and Fremont, \{Daved H.\} and Diamond, \{Michael S.\} and Whelan, \{Sean P.J.\}",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = mar,

day = "10",

doi = "10.1016/j.chom.2021.01.014",

language = "English",

volume = "29",

pages = "477--488.e4",

journal = "Cell Host and Microbe",

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Liu, Z, VanBlargan, LA, Bloyet, LM, Rothlauf, PW, Chen, RE, Stumpf, S, Zhao, H, Errico, JM, Theel, ES, Liebeskind, MJ, Alford, B, Buchser, WJ , Ellebedy, AH , Fremont, DH , Diamond, MS & Whelan, SPJ 2021, 'Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization', Cell Host and Microbe, vol. 29, no. 3, pp. 477-488.e4. https://doi.org/10.1016/j.chom.2021.01.014

TY - JOUR

T1 - Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization

AU - Liu, Zhuoming

AU - VanBlargan, Laura A.

AU - Bloyet, Louis Marie

AU - Rothlauf, Paul W.

AU - Chen, Rita E.

AU - Stumpf, Spencer

AU - Zhao, Haiyan

AU - Errico, John M.

AU - Theel, Elitza S.

AU - Liebeskind, Mariel J.

AU - Alford, Brynn

AU - Buchser, William J.

AU - Ellebedy, Ali H.

AU - Fremont, Daved H.

AU - Diamond, Michael S.

AU - Whelan, Sean P.J.

PY - 2021/3/10

Y1 - 2021/3/10

N2 - Liu et al. isolated 50 escape mutants to 19 monoclonal antibodies targeting the SARS-CoV-2 spike using a VSV-SARS2 chimera. Some mutants (S477N) resist neutralization by multiple mAbs, and others (E484K) are less sensitive to neutralization by convalescent sera. Several mutants exist in clinical SARS2-CoV-2 isolates and thus warrant further study.

AB - Liu et al. isolated 50 escape mutants to 19 monoclonal antibodies targeting the SARS-CoV-2 spike using a VSV-SARS2 chimera. Some mutants (S477N) resist neutralization by multiple mAbs, and others (E484K) are less sensitive to neutralization by convalescent sera. Several mutants exist in clinical SARS2-CoV-2 isolates and thus warrant further study.

KW - ACE2 receptor decoys

KW - COVID-19 vaccines

KW - SARS-CoV-2 escape mutants

KW - convalescent sera

KW - coronaviruses

KW - monoclonal antibodies

KW - receptor-binding domain

UR - https://www.scopus.com/pages/publications/85100439531

U2 - 10.1016/j.chom.2021.01.014

DO - 10.1016/j.chom.2021.01.014

M3 - Article

C2 - 33535027

AN - SCOPUS:85100439531

SN - 1931-3128

VL - 29

SP - 477-488.e4

JO - Cell Host and Microbe

JF - Cell Host and Microbe

IS - 3

ER -

Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization

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