Identification of QTL associated with resistance to Phytophthora fruit rot in cucumber (Cucumis sativus L.)

Ying Chen Lin, Ben N. Mansfeld, Xuemei Tang, Marivi Colle, Feifan Chen, Yiqun Weng, Zhangjun Fei, Rebecca Grumet

Research output: Contribution to journalArticlepeer-review

Abstract

Phytophthora fruit rot (PFR) caused by the soilborne oomycete pathogen, Phytophthora capsici, can cause severe yield loss in cucumber. With no resistant variety available, genetic resources are needed to develop resistant varieties. The goal of this work was to identify quantitative trait loci (QTL) associated with resistance to PFR using multiple genomic approaches and populations. Two types of resistances have been identified: age-related resistance (ARR) and young fruit resistance. ARR occurs at 12-16 days post pollination (dpp), coinciding with the end of exponential fruit growth. A major QTL for ARR was discovered on chromosome 3 and a candidate gene identified based on comparative transcriptomic analysis. Young fruit resistance, which is observed during the state of rapid fruit growth prior to commercial harvest, is a quantitative trait for which multiple QTL were identified. The largest effect QTL, qPFR5.1, located on chromosome 5 was fine mapped to a 1-Mb region. Genome-wide association studies (GWAS) and extreme-phenotype genome-wide association study (XP-GWAS) for young fruit resistance were also performed on a cucumber core collection representing > 96% of the genetic diversity of the USDA cucumber germplasm. Several SNPs overlapped with the QTL identified from QTL-seq analysis on biparental populations. In addition, novel SNPs associated with the resistance were identified from the germplasm. The resistant alleles were found mostly in accessions from India and South Asia, the center of diversity for cucumber. The results from this work can be applied to future disease resistance studies and marker-assisted selection in breeding programs.

Original languageEnglish
Article number1281755
JournalFrontiers in Plant Science
Volume14
DOIs
StatePublished - 2023

Keywords

  • age-related resistance
  • cucumber core collection
  • disease resistance
  • GWAS
  • Phytophthora capsici
  • QTL-seq
  • XP-GWAS

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