Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells

Sun Joo Lee; Hong Bae Jeon; Jeung Hwa Lee; Jong Shin Yoo; Jang Soo Chun; Yung Joon Yoo

doi:10.1016/S0014-5793(04)00243-1

Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells

Sun Joo Lee
, Hong Bae Jeon
, Jeung Hwa Lee
, Jong Shin Yoo
, Jang Soo Chun
, Yung Joon Yoo

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin-1, carbonic anhydrase-II (CA-II), 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase-2, and aldo-keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein-I (CRABP-I), CRABP-II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin-1, PAPS synthetase-2, and CA-II was regulated by extracellular signal-regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.

Original language	English
Pages (from-to)	35-40
Number of pages	6
Journal	FEBS Letters
Volume	563
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(04)00243-1
State	Published - Apr 9 2004

Keywords

AKR, aldo-keto reductase
CA-II, carbonic anhydrase-II
CRABP, cellular retinoic acid binding protein
Chondrocyte
Chondrogenesis
ECM, extracellular matrix
Extracellular matrix
FABP, fatty acid binding protein
Mesenchymal cell
Signaling pathway

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10.1016/S0014-5793(04)00243-1

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title = "Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells",

abstract = "We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin-1, carbonic anhydrase-II (CA-II), 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase-2, and aldo-keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein-I (CRABP-I), CRABP-II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin-1, PAPS synthetase-2, and CA-II was regulated by extracellular signal-regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.",

keywords = "AKR, aldo-keto reductase, CA-II, carbonic anhydrase-II, CRABP, cellular retinoic acid binding protein, Chondrocyte, Chondrogenesis, ECM, extracellular matrix, Extracellular matrix, FABP, fatty acid binding protein, Mesenchymal cell, Signaling pathway",

author = "Lee, \{Sun Joo\} and Jeon, \{Hong Bae\} and Lee, \{Jeung Hwa\} and Yoo, \{Jong Shin\} and Chun, \{Jang Soo\} and Yoo, \{Yung Joon\}",

note = "Funding Information: This work was supported by a grant (M1-0309-11-004) from the Systems Biology Program of the Korea Ministry of Science and Technology, by a K-JIST internal grant, and by the Brain Korea 21 Project of the Korean Ministry of Education.",

year = "2004",

month = apr,

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doi = "10.1016/S0014-5793(04)00243-1",

language = "English",

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pages = "35--40",

journal = "FEBS Letters",

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T1 - Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells

AU - Lee, Sun Joo

AU - Jeon, Hong Bae

AU - Lee, Jeung Hwa

AU - Yoo, Jong Shin

AU - Chun, Jang Soo

AU - Yoo, Yung Joon

N1 - Funding Information: This work was supported by a grant (M1-0309-11-004) from the Systems Biology Program of the Korea Ministry of Science and Technology, by a K-JIST internal grant, and by the Brain Korea 21 Project of the Korean Ministry of Education.

PY - 2004/4/9

Y1 - 2004/4/9

N2 - We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin-1, carbonic anhydrase-II (CA-II), 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase-2, and aldo-keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein-I (CRABP-I), CRABP-II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin-1, PAPS synthetase-2, and CA-II was regulated by extracellular signal-regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.

AB - We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin-1, carbonic anhydrase-II (CA-II), 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase-2, and aldo-keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein-I (CRABP-I), CRABP-II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin-1, PAPS synthetase-2, and CA-II was regulated by extracellular signal-regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.

KW - AKR, aldo-keto reductase

KW - CA-II, carbonic anhydrase-II

KW - CRABP, cellular retinoic acid binding protein

KW - Chondrocyte

KW - Chondrogenesis

KW - ECM, extracellular matrix

KW - Extracellular matrix

KW - FABP, fatty acid binding protein

KW - Mesenchymal cell

KW - Signaling pathway

UR - https://www.scopus.com/pages/publications/1842737760

U2 - 10.1016/S0014-5793(04)00243-1

DO - 10.1016/S0014-5793(04)00243-1

M3 - Article

C2 - 15063719

AN - SCOPUS:1842737760

SN - 0014-5793

VL - 563

SP - 35

EP - 40

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells

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Keywords

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