TY - JOUR
T1 - Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells
AU - Lee, Sun Joo
AU - Jeon, Hong Bae
AU - Lee, Jeung Hwa
AU - Yoo, Jong Shin
AU - Chun, Jang Soo
AU - Yoo, Yung Joon
N1 - Funding Information:
This work was supported by a grant (M1-0309-11-004) from the Systems Biology Program of the Korea Ministry of Science and Technology, by a K-JIST internal grant, and by the Brain Korea 21 Project of the Korean Ministry of Education.
PY - 2004/4/9
Y1 - 2004/4/9
N2 - We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin-1, carbonic anhydrase-II (CA-II), 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase-2, and aldo-keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein-I (CRABP-I), CRABP-II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin-1, PAPS synthetase-2, and CA-II was regulated by extracellular signal-regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.
AB - We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin-1, carbonic anhydrase-II (CA-II), 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase-2, and aldo-keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein-I (CRABP-I), CRABP-II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin-1, PAPS synthetase-2, and CA-II was regulated by extracellular signal-regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.
KW - AKR, aldo-keto reductase
KW - CA-II, carbonic anhydrase-II
KW - CRABP, cellular retinoic acid binding protein
KW - Chondrocyte
KW - Chondrogenesis
KW - ECM, extracellular matrix
KW - Extracellular matrix
KW - FABP, fatty acid binding protein
KW - Mesenchymal cell
KW - Signaling pathway
UR - http://www.scopus.com/inward/record.url?scp=1842737760&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(04)00243-1
DO - 10.1016/S0014-5793(04)00243-1
M3 - Article
C2 - 15063719
AN - SCOPUS:1842737760
SN - 0014-5793
VL - 563
SP - 35
EP - 40
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -