Identification of inosine and hypoxanthine as endogenous inhibitors of [3H] diazepam binding in the central nervous system

P. Skolnick, P. J. Marangos, F. K. Goodwin, M. Edwards, S. Paul

Research output: Contribution to journalArticlepeer-review

154 Scopus citations

Abstract

Endogenous inhibitors of [3H] diazepam binding have recently been isolated from bovine brain (1). These factors which competitively inhibit [3H] diazepam binding to synaptosomal membrane preparations have been characterized as dialyzable, heat stable, and resistant to degradation by proteolytic enzymes. Further purification by gel filtration, ion-exchange chromatography, thin-layer chromatography, ultraviolet spectroscopy and high pressure liquid chromatography demonstrate that these compounds are inosine and hypoxanthine.

Original languageEnglish
Pages (from-to)1473-1480
Number of pages8
JournalLife Sciences
Volume23
Issue number14
DOIs
StatePublished - Oct 9 1978
Externally publishedYes

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