Identification of hepatic peroxisomal phospholipase A2 and characterization of arachidonic acid-containing choline glycerophospholipids in hepatic peroxisomes

Jingyue Yang, Xianlin Han, Richard W. Gross

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Recently, a sequence encoding a novel mammalian calcium-independent phospholipase A2 (iPLA2γ) was identified in the human genome and subsequently cloned and expressed in Sf9 insect cells. Unexpectedly, expression studies in recombinant systems demonstrated the usage of multiple translation initiation codons resulting in different polypeptides. Herein, we demonstrate that hepatic iPLA2γ is localized to rat liver peroxisomes, possesses a molecular mass of 63 kDa and that peroxisomal membranes are highly enriched in arachidonic acid-containing phospholipids. Collectively, these results provide the first demonstration of iPLA2γ in mammalian tissue and suggest the possibility that iPLA2γ can contribute to lipid second messenger generation by hydrolysis of peroxisomal arachidonic acid-containing phospholipids.

Original languageEnglish
Pages (from-to)247-250
Number of pages4
JournalFEBS Letters
Volume546
Issue number2-3
DOIs
StatePublished - Jul 10 2003

Keywords

  • Arachidonic acid
  • Peroxisome
  • Phosphatidylcholine
  • Phospholipase A
  • Phospholipid
  • iPLAγ

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