@article{324f29eec4904d9cb72c740af3a1f056,
title = "Identification of Gut Bacteria such as Lactobacillus johnsonii that Disseminate to Systemic Tissues of Wild Type and MyD88–/– Mice",
abstract = "In healthy hosts the gut microbiota is restricted to gut tissues by several barriers some of which require MyD88-dependent innate immune sensor pathways. Nevertheless, some gut taxa have been reported to disseminate to systemic tissues. However, the extent to which this normally occurs during homeostasis in healthy organisms is still unknown. In this study, we recovered viable gut bacteria from systemic tissues of healthy wild type (WT) and MyD88−/− mice. Shotgun metagenomic-sequencing revealed a marked increase in the relative abundance of L. johnsonii in intestinal tissues of MyD88−/− mice compared to WT mice. Lactobacillus johnsonii was detected most frequently from multiple systemic tissues and at higher levels in MyD88−/− mice compared to WT mice. Viable L. johnsonii strains were recovered from different cell types sorted from intestinal and systemic tissues of WT and MyD88−/− mice. L. johnsonii could persist in dendritic cells and may represent murine immunomodulatory endosymbionts.",
keywords = "Gut microbiota, Lactobacillus johnsonii, MyD88, commensal bacteria, immunomodulation, symbionts, systemic dissemination, translocation",
author = "Sreeram Udayan and Panagiota Stamou and Fiona Crispie and Ana Hickey and Floyd, {Alexandria N.} and Hsieh, {Chyi Song} and Cotter, {Paul D.} and Orla O{\textquoteright}Sullivan and Silvia Melgar and O{\textquoteright}Toole, {Paul W.} and Newberry, {Rodney D.} and Valerio Rossini and Ken Nally",
note = "Funding Information: This work was supported by the Science Foundation Ireland under SFI Research Centre award [SFI-12/RC/2273] to APC Microbiome Ireland; National Institutes of Healthunder NIH grant [R01 DK097317] awarded to RDN. The authors would like to thank the staff at APC Microbiome Ireland Flow Cytometry Platform (UCC, Cork, Ireland), the Teagasc Next Generation DNA Sequencing Facility (Cork, Ireland) the APC Microbiome Ireland Germ-Free Platform (UCC, Cork, Ireland.) and the Washington University Gnotobiotic Research, Education and Transgenic (GREaT)” animal facility for their support with this project. Publisher Copyright: {\textcopyright} 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.",
year = "2022",
doi = "10.1080/19490976.2021.2007743",
language = "English",
volume = "14",
journal = "Gut microbes",
issn = "1949-0976",
number = "1",
}