Identification of factors that stimulate and inhibit growth of cultured human lens epithelial cells

J. R. Reddan, D. C. Dziedzic, T. P. Fleming, D. A. Smith, U. P. Andley

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Purpose: To optimize the medium and factors required for growth and prevention of cell death in transfected human lens epithelial cells (HLECs) (Andley et al., IOVS 35:3094). To identify factors inhibiting growth. Methods: 100,000 HLECs were grown in 7 different media containing 20%FBS or in MEM containing 0.1-20%FBS. Growth factors were added to MEM containing 0.1%FBS. Factors evaluated included, insulin, IGF-1, IGF-2, ECGS, EGF, FGFa, FGFb, HGF, PDGF-BB, KGF, IL6, SCF, TGFα and TGFβ1, TGFβ2 or TGFβ3. Cells were fed every other day and counted on day 7. Results: HLECs exhibited maximum growth in MEM with 20%FBS, died in MEM alone and exhibited limited growth in MEM supplemented with 0.1%FBS. Addition of any TGFβ at 50pg/ml to MEM+0.1%FBS inhibited growth. Cells cultured in ECGS (pituitary or neural) plus insulin, IGF-1 or IGF-2 exhibited a 3 fold increase over controls that exceeded growth obtained with other growth factor combinations. Addition of TGFβ1 to ECGS+IGF-1 inhibited proliferation. Individual factors that enhanced growth were insulin, IGF-1, IGF-2, ECGS, EGF, PDGF-BB, IL-6, SCF and TGFα. Conclusions: Optimization of medium and serum which stimulate growth and identification of growth factors that prevent death should help define the factors required for the long-term growth and survival of HLECs.

Original languageEnglish
Pages (from-to)S985
JournalInvestigative Ophthalmology and Visual Science
Issue number3
StatePublished - Feb 15 1996


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