TY - JOUR
T1 - Identification of factors that stimulate and inhibit growth of cultured human lens epithelial cells
AU - Reddan, J. R.
AU - Dziedzic, D. C.
AU - Fleming, T. P.
AU - Smith, D. A.
AU - Andley, U. P.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose: To optimize the medium and factors required for growth and prevention of cell death in transfected human lens epithelial cells (HLECs) (Andley et al., IOVS 35:3094). To identify factors inhibiting growth. Methods: 100,000 HLECs were grown in 7 different media containing 20%FBS or in MEM containing 0.1-20%FBS. Growth factors were added to MEM containing 0.1%FBS. Factors evaluated included, insulin, IGF-1, IGF-2, ECGS, EGF, FGFa, FGFb, HGF, PDGF-BB, KGF, IL6, SCF, TGFα and TGFβ1, TGFβ2 or TGFβ3. Cells were fed every other day and counted on day 7. Results: HLECs exhibited maximum growth in MEM with 20%FBS, died in MEM alone and exhibited limited growth in MEM supplemented with 0.1%FBS. Addition of any TGFβ at 50pg/ml to MEM+0.1%FBS inhibited growth. Cells cultured in ECGS (pituitary or neural) plus insulin, IGF-1 or IGF-2 exhibited a 3 fold increase over controls that exceeded growth obtained with other growth factor combinations. Addition of TGFβ1 to ECGS+IGF-1 inhibited proliferation. Individual factors that enhanced growth were insulin, IGF-1, IGF-2, ECGS, EGF, PDGF-BB, IL-6, SCF and TGFα. Conclusions: Optimization of medium and serum which stimulate growth and identification of growth factors that prevent death should help define the factors required for the long-term growth and survival of HLECs.
AB - Purpose: To optimize the medium and factors required for growth and prevention of cell death in transfected human lens epithelial cells (HLECs) (Andley et al., IOVS 35:3094). To identify factors inhibiting growth. Methods: 100,000 HLECs were grown in 7 different media containing 20%FBS or in MEM containing 0.1-20%FBS. Growth factors were added to MEM containing 0.1%FBS. Factors evaluated included, insulin, IGF-1, IGF-2, ECGS, EGF, FGFa, FGFb, HGF, PDGF-BB, KGF, IL6, SCF, TGFα and TGFβ1, TGFβ2 or TGFβ3. Cells were fed every other day and counted on day 7. Results: HLECs exhibited maximum growth in MEM with 20%FBS, died in MEM alone and exhibited limited growth in MEM supplemented with 0.1%FBS. Addition of any TGFβ at 50pg/ml to MEM+0.1%FBS inhibited growth. Cells cultured in ECGS (pituitary or neural) plus insulin, IGF-1 or IGF-2 exhibited a 3 fold increase over controls that exceeded growth obtained with other growth factor combinations. Addition of TGFβ1 to ECGS+IGF-1 inhibited proliferation. Individual factors that enhanced growth were insulin, IGF-1, IGF-2, ECGS, EGF, PDGF-BB, IL-6, SCF and TGFα. Conclusions: Optimization of medium and serum which stimulate growth and identification of growth factors that prevent death should help define the factors required for the long-term growth and survival of HLECs.
UR - http://www.scopus.com/inward/record.url?scp=24544448571&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:24544448571
SN - 0146-0404
VL - 37
SP - S985
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 3
ER -