TY - JOUR
T1 - Identification of Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the VITEK MS system
AU - Richter, S. S.
AU - Sercia, L.
AU - Branda, J. A.
AU - Burnham, C. A.D.
AU - Bythrow, M.
AU - Ferraro, M. J.
AU - Garner, O. B.
AU - Ginocchio, C. C.
AU - Jennemann, R.
AU - Lewinski, M. A.
AU - Manji, R.
AU - Mochon, A. B.
AU - Rychert, J. A.
AU - Westblade, L. F.
AU - Procop, G. W.
N1 - Funding Information:
Conflict of interest S.S. Richter has received a speaker honorarium from bioMérieux and research funding from bioMérieux, Nanosphere, Pocared, and Forest Laboratories. J.A. Branda, J.A. Rychert, and M.J. Ferraro have received research funding from bioMérieux and Becton Dickinson and Co. C.-A.D. Burnham has received research funding from bioMérieux, Accler8, Cepheid, and T2 Biosystems. C.C.
Funding Information:
Ginocchio has received research funding and consulting fees from bioMérieux and Becton Dickinson. M.A. Lewinski has received research funding from bioMérieux. G.W. Procop has received research funding from bioMérieux, Bruker, CDC, Pocared, and Luminex. All other authors declare that they have no conflict of interest.
PY - 2013/12
Y1 - 2013/12
N2 - This multicenter study evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry identifications from the VITEK MS system (bioMérieux, Marcy l'Etoile, France) for Enterobacteriaceae typically encountered in the clinical laboratory. Enterobacteriaceae isolates (n = 965) representing 17 genera and 40 species were analyzed on the VITEK MS system (database v2.0), in accordance with the manufacturer's instructions. Colony growth (≤72 h) was applied directly to the target slide. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry before mass spectrometry analysis. On the basis of the confidence level, the VITEK MS system provided a species, genus only, or no identification for each isolate. The accuracy of the mass spectrometric identification was compared to 16S rRNA gene sequencing performed at MIDI Labs (Newark, DE). Supplemental phenotypic testing was performed at bioMérieux when necessary. The VITEK MS result agreed with the reference method identification for 96.7 % of the 965 isolates tested, with 83.8 % correct to the species level and 12.8 % limited to a genus-level identification. There was no identification for 1.7 % of the isolates. The VITEK MS system misidentified 7 isolates (0.7 %) as different genera. Three Pantoea agglomerans isolates were misidentified as Enterobacter spp. and single isolates of Enterobacter cancerogenus, Escherichia hermannii, Hafnia alvei, and Raoultella ornithinolytica were misidentified as Klebsiella oxytoca, Citrobacter koseri, Obesumbacterium proteus, and Enterobacter aerogenes, respectively. Eight isolates (0.8 %) were misidentified as a different species in the correct genus. The VITEK MS system provides reliable mass spectrometric identifications for Enterobacteriaceae.
AB - This multicenter study evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry identifications from the VITEK MS system (bioMérieux, Marcy l'Etoile, France) for Enterobacteriaceae typically encountered in the clinical laboratory. Enterobacteriaceae isolates (n = 965) representing 17 genera and 40 species were analyzed on the VITEK MS system (database v2.0), in accordance with the manufacturer's instructions. Colony growth (≤72 h) was applied directly to the target slide. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry before mass spectrometry analysis. On the basis of the confidence level, the VITEK MS system provided a species, genus only, or no identification for each isolate. The accuracy of the mass spectrometric identification was compared to 16S rRNA gene sequencing performed at MIDI Labs (Newark, DE). Supplemental phenotypic testing was performed at bioMérieux when necessary. The VITEK MS result agreed with the reference method identification for 96.7 % of the 965 isolates tested, with 83.8 % correct to the species level and 12.8 % limited to a genus-level identification. There was no identification for 1.7 % of the isolates. The VITEK MS system misidentified 7 isolates (0.7 %) as different genera. Three Pantoea agglomerans isolates were misidentified as Enterobacter spp. and single isolates of Enterobacter cancerogenus, Escherichia hermannii, Hafnia alvei, and Raoultella ornithinolytica were misidentified as Klebsiella oxytoca, Citrobacter koseri, Obesumbacterium proteus, and Enterobacter aerogenes, respectively. Eight isolates (0.8 %) were misidentified as a different species in the correct genus. The VITEK MS system provides reliable mass spectrometric identifications for Enterobacteriaceae.
UR - http://www.scopus.com/inward/record.url?scp=84887989564&partnerID=8YFLogxK
U2 - 10.1007/s10096-013-1912-y
DO - 10.1007/s10096-013-1912-y
M3 - Article
C2 - 23818163
AN - SCOPUS:84887989564
SN - 0934-9723
VL - 32
SP - 1571
EP - 1578
JO - European Journal of Clinical Microbiology and Infectious Diseases
JF - European Journal of Clinical Microbiology and Infectious Diseases
IS - 12
ER -