Identification of Core Techniques That Enhance Genome Editing of Human T Cells Expressing Synthetic Antigen Receptors

Ju Fang Chang, Nils Wellhausen, Nils W. Engel, Jack H. Landmann, Caitlin R. Hopkins, January Salas-McKee, Adham S. Bear, Mehmet E. Selli, Sangya Agarwal, Julie K. Jadlowsky, Gerald P. Linette, Saar Gill, Carl H. June, Joseph A. Fraietta, Nathan Singh

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Genome editing technologies have seen remarkable progress in recent years, enabling precise regulation of exogenous and endogenous genes. These advances have been extensively applied to the engineering of human T lymphocytes, leading to the development of practice changing therapies for patients with cancer and the promise of synthetic immune cell therapies for a variety of nonmalignant diseases. Many distinct conceptual and technical approaches have been used to edit T-cell genomes, however targeted assessments of which techniques are most effective for manufacturing, gene editing, and transgene expression are rarely reported. Through extensive comparative evaluation, we identified methods that most effectively enhance engineering of research-scale and preclinical T-cell products at critical stages of manufacturing.

Original languageEnglish
Pages (from-to)1136-1146
Number of pages11
JournalCancer immunology research
Volume12
Issue number9
DOIs
StatePublished - Sep 1 2024

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