TY - JOUR
T1 - Identification of an unusual cyclooxygenase metabolite of arachidonic acid in rabbit renal medulla
AU - Morrison, Aubrey R.
AU - Crowley, Jan R.
N1 - Funding Information:
by NIH Research Grant R0130542 and Resource RROO954. Dr. Morrison is Investigator of the American Heart
PY - 1984/11/1
Y1 - 1984/11/1
N2 - A renal medulla 100,000g pellet metabolized arachidonic acid, C20:4, to the previously described prostaglandins prostaglandin E2, 6-ketoprostaglandin F1α, thromboxane B2, 12-hydroxyheptadecatrienoic acid, and 11-hydroxyeicosatetraenoic acid. In addition, under conditions of low enzyme to substrate ratios, the renal medulla also produced an unusual metabolite from arachidonic acid. This metabolite was inhibited by indomethacin, and thus suggested that it was a product of the cyclooxygenase. Addition of GSH to the incubation inhibited its formation, while p-hydroxymercuri-benzoate enhanced its formation. This compound was identified by HPLC purification, uv absorption, and gas chromatography-mass spectroscopy. The compound was 9,15 dioxo,11-hydroxyprosta-5,13-dienoic acid.
AB - A renal medulla 100,000g pellet metabolized arachidonic acid, C20:4, to the previously described prostaglandins prostaglandin E2, 6-ketoprostaglandin F1α, thromboxane B2, 12-hydroxyheptadecatrienoic acid, and 11-hydroxyeicosatetraenoic acid. In addition, under conditions of low enzyme to substrate ratios, the renal medulla also produced an unusual metabolite from arachidonic acid. This metabolite was inhibited by indomethacin, and thus suggested that it was a product of the cyclooxygenase. Addition of GSH to the incubation inhibited its formation, while p-hydroxymercuri-benzoate enhanced its formation. This compound was identified by HPLC purification, uv absorption, and gas chromatography-mass spectroscopy. The compound was 9,15 dioxo,11-hydroxyprosta-5,13-dienoic acid.
UR - http://www.scopus.com/inward/record.url?scp=0021722739&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(84)90287-X
DO - 10.1016/0003-9861(84)90287-X
M3 - Article
C2 - 6437329
AN - SCOPUS:0021722739
SN - 0003-9861
VL - 234
SP - 413
EP - 417
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -