Identification of alterations in DNA copy number in host stromal cell during tumor progression

Robert J. Pelham, Linda Rodgers, Ira Hall, Robert Lucito, Ken C.Q. Nguyen, Nicholas Navin, James Hicks, David Mu, Scott Powers, Michael Wigler, David Botstein

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The interactions between cancer cells and the surrounding host stromal tissue play a critical role in tumor progression and metastasis, but the molecular nature of this relationship remains largely uncharacterized. Furthermore, although genetic changes of neoplastic cells in tumors contribute significantly to tumor progression, it is not known whether similar changes occur in the adjacent host stromal microenvironment and whether they contribute to or inhibit tumorigenesis. To address this question in an unbiased and genome-wide manner, we applied high-resolution DNA copy number analysis to murine stromal DNA isolated from human xenograft tumors that were formed in immunodeficient mice. We show that numerous amplifications and deletions are found within the host stromal microenvironment, suggesting that alterations in host DNA copy number can occur and may play a significant role in modifying tumor-stromal interactions.

Original languageEnglish
Pages (from-to)19848-19853
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number52
DOIs
StatePublished - Dec 26 2006

Keywords

  • Cancer
  • Microarray
  • Stroma

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