Identification of adult midgut precursors in Drosophila

Craig A. Micchelli, Lisa Sudmeier, Norbert Perrimon, Shan Tang, Ryan Beehler-Evans

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

The adult Drosophila midgut is thought to arise from an endodermal rudiment specified during embryogenesis. Previous studies have reported the presence of individual cells termed adult midgut precursors (AMPs) as well as "midgut islands" or "islets" in embryonic and larval midgut tissue. Yet the precise relationship between progenitor cell populations and the cells of the adult midgut has not been characterized. Using a combination of molecular markers and directed cell lineage tracing, we provide evidence that the adult midgut arises from a molecularly distinct population of single cells present by the embryonic/larval transition. AMPs reside in a distinct basal position in the larval midgut where they remain through all subsequent larval and pupal stages and into adulthood. At least five phases of AMP activity are associated with the stepwise process of midgut formation. Our data shows that during larval stages AMPs give rise to the presumptive adult epithelium; during pupal stages AMPs contribute to the final size, cell number and form. Finally, a genetic screen has led to the identification of the Ecdysone receptor as a regulator of AMP expansion.

Original languageEnglish
Pages (from-to)12-21
Number of pages10
JournalGene Expression Patterns
Volume11
Issue number1-2
DOIs
StatePublished - Jan 2011

Keywords

  • Adult midgut precursors
  • Drosophila
  • Ecdysone
  • Intestinal stem cell

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