We have chemically analyzed the peptides presented by I-Ek molecules after processing of hen egg lysozyme (HEL) by a murine B-lymphoma line or by splenocytes. In both cases, the identified peptides were derived from a single region of HEL, containing the core residues 85-96 with heterogeneous N and C termini. This was a surprising result because this determinant had previously been described as cryptic - i.e., not presented after processing of intact HEL. Examination of the specificities of T hybridomas isolated after immunization with either HEL or 84-96 peptide (p84-96) provided an explanation for this controversy. Whereas hybridomas induced by immunization with HEL responded equally well to HEL and p84-96, those induced by peptide immunization showed a marked preference for p84-96 over intact HEL. In other words, hybridomas isolated after p84-96 immunization responded poorly to forms of the 84-96 determinant produced by natural processing, leading to the possible erroneous interpretation that 84-96 is a hidden determinant. We conclude that (i) p84-96 is efficiently presented on I-Ek molecules after processing of HEL, (ii) the explanation for the weak lymph node response to this epitope after immunization with HEL lies at the level of the T cell, not the antigen-presenting cell, and (iii) crypticity cannot be defined on the basis of T-cell proliferation studies alone.
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Mar 14 1995|
- Antigen processing
- Major histocompatibility complex
- Peptide immunization
- Peptide sequencing