Identification and molecular characterization of a novel splice variant of human 5- lipoxygenase gene

Abhay Kumar Singh, Rahul Kumar, Abhay Anand Shukla, Gururao Hariprasad, Shyam Sunder Chauhan, Sharmistha Dey

Research output: Contribution to journalArticlepeer-review

Abstract

5-Lipoxygenase (5-LO) is one of the members of Lipoxygenase family. It breaks down arachidonic acid to pro-inflammatory compounds like leukotrienes. Leukotriene plays a major role in the inflammatory process. In this study, while cloning full length 5-LO, a novel splice variant of 5-LO (t5-LO) was found to be expressed in HepG2 cell line. The complete ORF of t5-LO is 420 bp long, expressing 139 amino acid long proteins from N-terminal. The splice variant of 5-LO was cloned, expressed, purified in bacterial system and characterized by MS/MS and western blot experiments. The full length 5-LO is 674 amino acids long encoded by 2,025 bp long ORF. RT-PCR and western blot revealed that t5-LO is extensively expressed in HepG2 cell line.

Original languageEnglish
Pages (from-to)8255-8260
Number of pages6
JournalMolecular Biology Reports
Volume41
Issue number12
DOIs
StatePublished - Dec 1 2014

Keywords

  • 5-Lipooxygenase
  • Human
  • Real time PCR
  • Splice variant

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