Abstract
5-Lipoxygenase (5-LO) is one of the members of Lipoxygenase family. It breaks down arachidonic acid to pro-inflammatory compounds like leukotrienes. Leukotriene plays a major role in the inflammatory process. In this study, while cloning full length 5-LO, a novel splice variant of 5-LO (t5-LO) was found to be expressed in HepG2 cell line. The complete ORF of t5-LO is 420 bp long, expressing 139 amino acid long proteins from N-terminal. The splice variant of 5-LO was cloned, expressed, purified in bacterial system and characterized by MS/MS and western blot experiments. The full length 5-LO is 674 amino acids long encoded by 2,025 bp long ORF. RT-PCR and western blot revealed that t5-LO is extensively expressed in HepG2 cell line.
Original language | English |
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Pages (from-to) | 8255-8260 |
Number of pages | 6 |
Journal | Molecular Biology Reports |
Volume | 41 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1 2014 |
Keywords
- 5-Lipooxygenase
- Human
- Real time PCR
- Splice variant