Identification and localization of the CupB protein involved in constitutive CO2 uptake in the cyanobacterium, Synechocystis sp. strain PCC 6803

Min Xu; Teruo Ogawa; Himadri B. Pakrasi; Hualing Mi

doi:10.1093/pcp/pcn074

Identification and localization of the CupB protein involved in constitutive CO₂ uptake in the cyanobacterium, Synechocystis sp. strain PCC 6803

Min Xu
, Teruo Ogawa
, Himadri B. Pakrasi
, Hualing Mi

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Antibody against cMyc cross-reacted strongly with the CupB protein tagged with His6-cMyc (HM) in thylakoid membrane of Synechocystis sp. strain PCC 6803 but only faintly with the cytoplasmic membrane fraction. The protein was not detected in the membranes of the ΔndhD4 and ΔndhF4 mutants in which CupB was tagged with HM. We concluded that a CupB complex containing NdhD4 and NdhF4 is largely, if not exclusively, confined to the thylakoid membrane. Both CupB and NdhH were detected in a fraction containing protein complexes of > 450 kDa, obtained after nickel column and gel filtration chromatography of the membranes solubilized with n-dodecyl-β-maltoside.

Original language	English
Pages (from-to)	994-997
Number of pages	4
Journal	Plant and Cell Physiology
Volume	49
Issue number	6
DOIs	https://doi.org/10.1093/pcp/pcn074
State	Published - Jun 2008

Keywords

CO uptake
CupB
Cytoplasmic membrane
NDH-1
Synechocystis 6803
Thylakoid membrane

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10.1093/pcp/pcn074

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title = "Identification and localization of the CupB protein involved in constitutive CO2 uptake in the cyanobacterium, Synechocystis sp. strain PCC 6803",

abstract = "Antibody against cMyc cross-reacted strongly with the CupB protein tagged with His6-cMyc (HM) in thylakoid membrane of Synechocystis sp. strain PCC 6803 but only faintly with the cytoplasmic membrane fraction. The protein was not detected in the membranes of the ΔndhD4 and ΔndhF4 mutants in which CupB was tagged with HM. We concluded that a CupB complex containing NdhD4 and NdhF4 is largely, if not exclusively, confined to the thylakoid membrane. Both CupB and NdhH were detected in a fraction containing protein complexes of > 450 kDa, obtained after nickel column and gel filtration chromatography of the membranes solubilized with n-dodecyl-β-maltoside.",

keywords = "CO uptake, CupB, Cytoplasmic membrane, NDH-1, Synechocystis 6803, Thylakoid membrane",

author = "Min Xu and Teruo Ogawa and Pakrasi, \{Himadri B.\} and Hualing Mi",

note = "Funding Information: The National Natural Science Foundation of China (No. 306701620 to H.M.); the State Key Basic Research and Development Plan (No. 20007CB108804 to H.M.), the Key foundation of the Chinese Academy of Science (No. KSCX2-YW-N-059 to H.M.); the Membrane Biology Environmental Molecular Science Laboratory Scientific Grand Challenge Project at the W. R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the US Department of Energy Office of Biological and Environmental Research program located at Pacific Northwest National Laboratory (to T.O. and H.P.). Pacific Northwest National Laboratory is operated for the Department of Energy by Battelle.",

year = "2008",

month = jun,

doi = "10.1093/pcp/pcn074",

language = "English",

volume = "49",

pages = "994--997",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

number = "6",

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T1 - Identification and localization of the CupB protein involved in constitutive CO2 uptake in the cyanobacterium, Synechocystis sp. strain PCC 6803

AU - Xu, Min

AU - Ogawa, Teruo

AU - Pakrasi, Himadri B.

AU - Mi, Hualing

N1 - Funding Information: The National Natural Science Foundation of China (No. 306701620 to H.M.); the State Key Basic Research and Development Plan (No. 20007CB108804 to H.M.), the Key foundation of the Chinese Academy of Science (No. KSCX2-YW-N-059 to H.M.); the Membrane Biology Environmental Molecular Science Laboratory Scientific Grand Challenge Project at the W. R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the US Department of Energy Office of Biological and Environmental Research program located at Pacific Northwest National Laboratory (to T.O. and H.P.). Pacific Northwest National Laboratory is operated for the Department of Energy by Battelle.

PY - 2008/6

Y1 - 2008/6

N2 - Antibody against cMyc cross-reacted strongly with the CupB protein tagged with His6-cMyc (HM) in thylakoid membrane of Synechocystis sp. strain PCC 6803 but only faintly with the cytoplasmic membrane fraction. The protein was not detected in the membranes of the ΔndhD4 and ΔndhF4 mutants in which CupB was tagged with HM. We concluded that a CupB complex containing NdhD4 and NdhF4 is largely, if not exclusively, confined to the thylakoid membrane. Both CupB and NdhH were detected in a fraction containing protein complexes of > 450 kDa, obtained after nickel column and gel filtration chromatography of the membranes solubilized with n-dodecyl-β-maltoside.

AB - Antibody against cMyc cross-reacted strongly with the CupB protein tagged with His6-cMyc (HM) in thylakoid membrane of Synechocystis sp. strain PCC 6803 but only faintly with the cytoplasmic membrane fraction. The protein was not detected in the membranes of the ΔndhD4 and ΔndhF4 mutants in which CupB was tagged with HM. We concluded that a CupB complex containing NdhD4 and NdhF4 is largely, if not exclusively, confined to the thylakoid membrane. Both CupB and NdhH were detected in a fraction containing protein complexes of > 450 kDa, obtained after nickel column and gel filtration chromatography of the membranes solubilized with n-dodecyl-β-maltoside.

KW - CO uptake

KW - CupB

KW - Cytoplasmic membrane

KW - NDH-1

KW - Synechocystis 6803

KW - Thylakoid membrane

UR - https://www.scopus.com/pages/publications/48849109157

U2 - 10.1093/pcp/pcn074

DO - 10.1093/pcp/pcn074

M3 - Article

C2 - 18467341

AN - SCOPUS:48849109157

SN - 0032-0781

VL - 49

SP - 994

EP - 997

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 6

ER -

Identification and localization of the CupB protein involved in constitutive CO₂ uptake in the cyanobacterium, Synechocystis sp. strain PCC 6803

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