Hyperacute rejection after portacaval shunt in the rat

G. Mazzoni, P. Mazzoni

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The present study was performed to assess the influence of PCS on the rejection reaction cascade of the heart-lung allograft (HLA) between two strains of rats with a strong histocompatibility barrier. These experiments are consistent with two correlated results: first, an accelerated hyperacute rejection of the graft in the animals who underwent PCS, the median survival time of the graft was 5.7 days v the 7.9 days of grafted animals without PCS. Second, a striking modification of the peripheral lymphocyte phenotype in PCS was obtained; TT cells, as well as Th and Ts, increased steadily after PCS, particularly the Ts cells. The H/S ratio was maintained above 2 (2.8). The study of subset T cells in the grafted animals with PCS showed an increase of Tc/s cells in 80% of the cases starting immediately after surgery; Th remained unchanged or slightly increased. The Th/Tc/s was 0.7 at the time of rejection. In the group of animals transplanted without PCS, Th subset cells decreased starting on the fourth POD; Ts cells remained unchanged until the fourth POD and then progressively increased during rejection. H/S ratio was 1.7 at POD. In our experiment we documented an accelerated rejection when the animal underwent PCS. The H/S ratio was completely inverted. Ts cells were already elevated in the first POD. Suppressor T cells may play the role of a mediator of direct lymphocytotoxicity in the hyperacute rejection of the graft. The accelerated rejection reaction after PCS can be the result of a precocious and uncontrolled stimulus of antigen contained with portal blood to the lymphatic tissue, including the spleen. The animals with PCS may produce, during the first few days after surgery, circulating cytotoxic antibodies responsible for precipitating and accelerating the rejection reaction when we challenge the shunted animals with heart lung graft.

Original languageEnglish
Pages (from-to)844-847
Number of pages4
JournalTransplantation Proceedings
Issue number1 SUPPL. 1
StatePublished - Jan 1 1988


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