Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT

C. N. Abboud; J. K. Brennan; G. H. Barlow; M. A. Lichtman

doi:10.1182/blood.v58.6.1148.bloodjournal5861148

Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT

C. N. Abboud
, J. K. Brennan
, G. H. Barlow
, M. A. Lichtman

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The human cell line, GCT, secretes hemopoietins into serum-free culture medium. The conditioned medium contains activities that stimulate neutrophil-monocyte, macrophage, eosinophil, and erythroid colony growth in human marrow cultures. We have used hydrophobic adsorption chromatography to separate a neutrophil-monocyte colony-stimulating activities. This hydrophobic CSF has no eosinophil-stimulating activity and is virtually devoid of erythroid-stimulating activity.

Original language	English
Pages (from-to)	1148-1154
Number of pages	7
Journal	Blood
Volume	58
Issue number	6
DOIs	https://doi.org/10.1182/blood.v58.6.1148.bloodjournal5861148
State	Published - 1981

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title = "Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT",

abstract = "The human cell line, GCT, secretes hemopoietins into serum-free culture medium. The conditioned medium contains activities that stimulate neutrophil-monocyte, macrophage, eosinophil, and erythroid colony growth in human marrow cultures. We have used hydrophobic adsorption chromatography to separate a neutrophil-monocyte colony-stimulating activities. This hydrophobic CSF has no eosinophil-stimulating activity and is virtually devoid of erythroid-stimulating activity.",

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AU - Abboud, C. N.

AU - Brennan, J. K.

AU - Barlow, G. H.

AU - Lichtman, M. A.

PY - 1981

Y1 - 1981

N2 - The human cell line, GCT, secretes hemopoietins into serum-free culture medium. The conditioned medium contains activities that stimulate neutrophil-monocyte, macrophage, eosinophil, and erythroid colony growth in human marrow cultures. We have used hydrophobic adsorption chromatography to separate a neutrophil-monocyte colony-stimulating activities. This hydrophobic CSF has no eosinophil-stimulating activity and is virtually devoid of erythroid-stimulating activity.

AB - The human cell line, GCT, secretes hemopoietins into serum-free culture medium. The conditioned medium contains activities that stimulate neutrophil-monocyte, macrophage, eosinophil, and erythroid colony growth in human marrow cultures. We have used hydrophobic adsorption chromatography to separate a neutrophil-monocyte colony-stimulating activities. This hydrophobic CSF has no eosinophil-stimulating activity and is virtually devoid of erythroid-stimulating activity.

UR - https://www.scopus.com/pages/publications/0019848272

U2 - 10.1182/blood.v58.6.1148.bloodjournal5861148

DO - 10.1182/blood.v58.6.1148.bloodjournal5861148

M3 - Article

C2 - 6975643

AN - SCOPUS:0019848272

SN - 0006-4971

VL - 58

SP - 1148

EP - 1154

JO - Blood

JF - Blood

IS - 6

ER -

Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT

Abstract

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