Hybrid reference genome assembly for the archetypal Leishmania (Viannia) guyanensis bearing the dsRNA totivirus LRV1

Peter J. Myler; Gowthaman Ramasamy; Wesley C. Warren; Deborah E. Dobson; Stephen M. Beverley

doi:10.1128/mra.00992-22

Hybrid reference genome assembly for the archetypal Leishmania (Viannia) guyanensis bearing the dsRNA totivirus LRV1

Peter J. Myler
, Gowthaman Ramasamy
, Wesley C. Warren
, Deborah E. Dobson
, Stephen M. Beverley

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We report a high-quality hybrid genome assembly and annotation for a Leishmania (Viannia) guyanensis line (M4147) expressing a firefly luciferase reporter gene and bearing the totivirus LRV1 strongly implicated in parasite hypervirulence. This reference genome should facilitate the study of infectivity and pathogenesis of cutaneous and severe leishmaniasis.

Original language	English
Journal	Microbiology Resource Announcements
Volume	14
Issue number	8
DOIs	https://doi.org/10.1128/mra.00992-22
State	Published - Aug 2025

Keywords

Leishmania guyanensis
South America
Trypanosomatidae
Viannia
comparative genomics
kinetoplastida
mucocutaneous leishmaniasis

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10.1128/mra.00992-22

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title = "Hybrid reference genome assembly for the archetypal Leishmania (Viannia) guyanensis bearing the dsRNA totivirus LRV1",

abstract = "We report a high-quality hybrid genome assembly and annotation for a Leishmania (Viannia) guyanensis line (M4147) expressing a firefly luciferase reporter gene and bearing the totivirus LRV1 strongly implicated in parasite hypervirulence. This reference genome should facilitate the study of infectivity and pathogenesis of cutaneous and severe leishmaniasis.",

keywords = "Leishmania guyanensis, South America, Trypanosomatidae, Viannia, comparative genomics, kinetoplastida, mucocutaneous leishmaniasis",

author = "Myler, \{Peter J.\} and Gowthaman Ramasamy and Warren, \{Wesley C.\} and Dobson, \{Deborah E.\} and Beverley, \{Stephen M.\}",

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AU - Myler, Peter J.

AU - Ramasamy, Gowthaman

AU - Warren, Wesley C.

AU - Dobson, Deborah E.

AU - Beverley, Stephen M.

PY - 2025/8

Y1 - 2025/8

N2 - We report a high-quality hybrid genome assembly and annotation for a Leishmania (Viannia) guyanensis line (M4147) expressing a firefly luciferase reporter gene and bearing the totivirus LRV1 strongly implicated in parasite hypervirulence. This reference genome should facilitate the study of infectivity and pathogenesis of cutaneous and severe leishmaniasis.

AB - We report a high-quality hybrid genome assembly and annotation for a Leishmania (Viannia) guyanensis line (M4147) expressing a firefly luciferase reporter gene and bearing the totivirus LRV1 strongly implicated in parasite hypervirulence. This reference genome should facilitate the study of infectivity and pathogenesis of cutaneous and severe leishmaniasis.

KW - Leishmania guyanensis

KW - South America

KW - Trypanosomatidae

KW - Viannia

KW - comparative genomics

KW - kinetoplastida

KW - mucocutaneous leishmaniasis

UR - https://www.scopus.com/pages/publications/105013372734

U2 - 10.1128/mra.00992-22

DO - 10.1128/mra.00992-22

M3 - Article

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AN - SCOPUS:105013372734

SN - 2576-098X

VL - 14

JO - Microbiology Resource Announcements

JF - Microbiology Resource Announcements

IS - 8

ER -

Hybrid reference genome assembly for the archetypal Leishmania (Viannia) guyanensis bearing the dsRNA totivirus LRV1

Abstract

Keywords

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