Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro

Chuda Chittasupho; Joshua Sestak; Laura Shannon; Teruna J. Siahaan; Charlotte M. Vines; Cory Berkland

doi:10.1021/mp4003909

Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro

Chuda Chittasupho
, Joshua Sestak
, Laura Shannon
, Teruna J. Siahaan
, Charlotte M. Vines
, Cory Berkland

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A novel oxime grafting scheme was utilized to conjugate an ICAM-1 ligand (LABL), a cellular antigen ovalbumin (OVA), or both peptides simultaneously to hyaluronic acid (HA). Samples of HA only and the various peptide grafted HA were found to bind to dendritic cells (DCs). HA with grafted LABL and OVA showed the greatest binding to DCs. Dendritic cells treated with HA, HA with grafted LABL, or HA with grafted LABL and OVA significantly suppressed T cell and DC conjugate formation and T cell proliferation and reduced proinflammatory cytokine production compared to untreated cells. These results suggest that HA serves as an effective backbone for multivalent ligand presentation for inhibiting T cell response to antigen presentation. In addition, multivalent display of both antigen and an ICAM-1 inhibitor (LABL) may enhance binding to DCs and could potentially disrupt cellular signaling leading to autoimmunity.

Original language	English
Pages (from-to)	367-373
Number of pages	7
Journal	Molecular Pharmaceutics
Volume	11
Issue number	1
DOIs	https://doi.org/10.1021/mp4003909
State	Published - Jan 6 2014

Keywords

dendritic cells
hyaluronic acid
peptides
T cells
targeted delivery

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10.1021/mp4003909

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abstract = "A novel oxime grafting scheme was utilized to conjugate an ICAM-1 ligand (LABL), a cellular antigen ovalbumin (OVA), or both peptides simultaneously to hyaluronic acid (HA). Samples of HA only and the various peptide grafted HA were found to bind to dendritic cells (DCs). HA with grafted LABL and OVA showed the greatest binding to DCs. Dendritic cells treated with HA, HA with grafted LABL, or HA with grafted LABL and OVA significantly suppressed T cell and DC conjugate formation and T cell proliferation and reduced proinflammatory cytokine production compared to untreated cells. These results suggest that HA serves as an effective backbone for multivalent ligand presentation for inhibiting T cell response to antigen presentation. In addition, multivalent display of both antigen and an ICAM-1 inhibitor (LABL) may enhance binding to DCs and could potentially disrupt cellular signaling leading to autoimmunity.",

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T1 - Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro

AU - Chittasupho, Chuda

AU - Sestak, Joshua

AU - Shannon, Laura

AU - Siahaan, Teruna J.

AU - Vines, Charlotte M.

AU - Berkland, Cory

PY - 2014/1/6

Y1 - 2014/1/6

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AB - A novel oxime grafting scheme was utilized to conjugate an ICAM-1 ligand (LABL), a cellular antigen ovalbumin (OVA), or both peptides simultaneously to hyaluronic acid (HA). Samples of HA only and the various peptide grafted HA were found to bind to dendritic cells (DCs). HA with grafted LABL and OVA showed the greatest binding to DCs. Dendritic cells treated with HA, HA with grafted LABL, or HA with grafted LABL and OVA significantly suppressed T cell and DC conjugate formation and T cell proliferation and reduced proinflammatory cytokine production compared to untreated cells. These results suggest that HA serves as an effective backbone for multivalent ligand presentation for inhibiting T cell response to antigen presentation. In addition, multivalent display of both antigen and an ICAM-1 inhibitor (LABL) may enhance binding to DCs and could potentially disrupt cellular signaling leading to autoimmunity.

KW - dendritic cells

KW - hyaluronic acid

KW - peptides

KW - T cells

KW - targeted delivery

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Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro

Abstract

Keywords

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