TY - JOUR
T1 - Human U2B″ protein binding to snRNA stemloops
AU - Williams, Sandra G.
AU - Hall, Kathleen B.
PY - 2011/11
Y1 - 2011/11
N2 - The human U2B″ protein is one of the unique proteins that comprise the U2 snRNP, but it is also a representative of the U1A/U2B″ protein family. In the U2 snRNP, it is bound to Stem-Loop IV (SLIV) of the U2 snRNA. We find that in vitro it binds not only to human SLIV, but also to Stem-Loop II (SLII) from human U1 snRNA and to Drosophila U2 snRNA SLIV. The thermodynamics of these binding interactions show a striking similarity, leading to the conclusion that U2B″ has a relaxed specificity for its RNA targets. The binding properties of U2B″ are distinct from those of human U1A and of Drosophila SNF, despite its high homology to those proteins, and so provide important new information on how this protein family has modulated its target preferences.
AB - The human U2B″ protein is one of the unique proteins that comprise the U2 snRNP, but it is also a representative of the U1A/U2B″ protein family. In the U2 snRNP, it is bound to Stem-Loop IV (SLIV) of the U2 snRNA. We find that in vitro it binds not only to human SLIV, but also to Stem-Loop II (SLII) from human U1 snRNA and to Drosophila U2 snRNA SLIV. The thermodynamics of these binding interactions show a striking similarity, leading to the conclusion that U2B″ has a relaxed specificity for its RNA targets. The binding properties of U2B″ are distinct from those of human U1A and of Drosophila SNF, despite its high homology to those proteins, and so provide important new information on how this protein family has modulated its target preferences.
KW - Protein:RNA thermodynamics
KW - RNA binding protein
KW - RRM
KW - U2B″
UR - http://www.scopus.com/inward/record.url?scp=80052305240&partnerID=8YFLogxK
U2 - 10.1016/j.bpc.2011.05.010
DO - 10.1016/j.bpc.2011.05.010
M3 - Article
C2 - 21684671
AN - SCOPUS:80052305240
SN - 0301-4622
VL - 159
SP - 82
EP - 89
JO - Biophysical Chemistry
JF - Biophysical Chemistry
IS - 1
ER -