Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning

Brian A. Van Tine, Luan D. Dao, Shwu Yuan Wu, Timothy M. Sonbuchner, Biing Yuan Lin, Nianxiang Zou, Cheng Ming Chiang, Thomas R. Broker, Louise T. Chow

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

Human papillomaviruses (HPVs) establish long-term infections in patients. The mechanism for extrachromosomal HPV DNA persistence in cycling cells is unknown. We show that HPV origin-containing plasmids partition as minichromosomes, attributable to an association of the viral origin recognition protein E2 with mitotic spindles. α, β-, and γ-tubulins were pulled down with a tagged E2. The N-terminal transacting and C-terminal protein dimerization/DNA binding domains independently associated with the spindles. We suggest that this E2 property enables these viruses to establish persistence. Its implication for HPV oncogenesis is presented.

Original languageEnglish
Pages (from-to)4030-4035
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number12
DOIs
StatePublished - Mar 23 2004
Externally publishedYes

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