Abstract
A cDNA clone encoding human pancreatic procolipase was incorporated into a recombinant baculovirus. Spodoptera frugiperda insect cells infected with the recombinant baculovirus secreted procolipase into the medium, which could be isolated in a single step by immunoaffinity chromatography. The highly purified protein reactivated human pancreatic lipase in a concentration-dependent fashion and was efficiently converted to colipase by limited trypsin digestion. This expression system is suitable for producing amounts of procolipase adequate for biophysical studies.
| Original language | English |
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| Pages (from-to) | 583-586 |
| Number of pages | 4 |
| Journal | Protein Expression and Purification |
| Volume | 5 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1994 |