Human cell lines that elaborate colony stimulating activity for the marrow cells of man and other species

J. F. Di Persio, J. K. Brennan, M. A. Lichtman, B. L. Speiser

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

We have established two-human cell lines which elaborate colony-stimulating activity (CSA) for at least four species: man, mouse, rabbit, and dog. One GCT, was isolated from a lung metastasis of a fibrous histiocytoma; the other RC4, from a monocyte-enriched fraction of normal blood. Medium conditioned by either GCT or RC4 cells was more potent in stimulating human marrow growth in vitro than was monocyte-conditioned medium or human leukocyte feeder layers. Fractionation of cell line-conditioned medium by Sephacryl S-200 chromatography indicated that the maximum activity of the CSA for human marrow cells is eluted within the range of 30,000-40,000 daltons. These cell lines provide a continuous source of large quantities of conditioned medium for purification of CSA. Moreover, the invariable growth-supporting activity for all species tested and the high potency of cell-line CSA facilitates studies of its elaboration and biologic effects.

Original languageEnglish
Pages (from-to)507-519
Number of pages13
JournalBlood
Volume51
Issue number3
DOIs
StatePublished - 1978

Fingerprint

Dive into the research topics of 'Human cell lines that elaborate colony stimulating activity for the marrow cells of man and other species'. Together they form a unique fingerprint.

Cite this