Human Cardiac Pericytes Are Susceptible to SARS-CoV-2 Infection

Brittany D. Brumback; Oleksandr Dmytrenko; Ashley N. Robinson; Adam L. Bailey; Pan Ma; Jing Liu; Stephanie C. Hicks; Sherwin Ng; Gang Li; David M. Zhang; Catherine E. Lipovsky; Chieh Yu Lin; Michael S. Diamond; Kory J. Lavine; Stacey L. Rentschler

doi:10.1016/j.jacbts.2022.09.001

Human Cardiac Pericytes Are Susceptible to SARS-CoV-2 Infection

Brittany D. Brumback, Oleksandr Dmytrenko, Ashley N. Robinson, Adam L. Bailey, Pan Ma, Jing Liu, Stephanie C. Hicks, Sherwin Ng, Gang Li, David M. Zhang, Catherine E. Lipovsky, Chieh Yu Lin, Michael S. Diamond, Kory J. Lavine, Stacey L. Rentschler

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

COVID-19 is associated with serious cardiovascular complications, with incompletely understood mechanism(s). Pericytes have key functions in supporting endothelial cells and maintaining vascular integrity. We demonstrate that human cardiac pericytes are permissive to SARS-CoV-2 infection in organotypic slice and primary cell cultures. Viral entry into pericytes is mediated by endosomal proteases, and infection leads to up-regulation of inflammatory markers, vasoactive mediators, and nuclear factor kappa-B–dependent cell death. Furthermore, we present evidence of cardiac pericyte infection in COVID-19 myocarditis patients. These data demonstrate that human cardiac pericytes are susceptible to SARS-CoV-2 infection and suggest a role for pericyte infection in COVID-19.

Original language	English
Pages (from-to)	109-120
Number of pages	12
Journal	JACC: Basic to Translational Science
Volume	8
Issue number	2
DOIs	https://doi.org/10.1016/j.jacbts.2022.09.001
State	Published - Feb 2023

Keywords

COVID-19
SARS-CoV-2
cardiovascular disease
pericytes

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10.1016/j.jacbts.2022.09.001

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Brumback, B. D., Dmytrenko, O., Robinson, A. N., Bailey, A. L., Ma, P., Liu, J., Hicks, S. C., Ng, S., Li, G., Zhang, D. M., Lipovsky, C. E., Lin, C. Y., Diamond, M. S., Lavine, K. J., & Rentschler, S. L. (2023). Human Cardiac Pericytes Are Susceptible to SARS-CoV-2 Infection. JACC: Basic to Translational Science, 8(2), 109-120. https://doi.org/10.1016/j.jacbts.2022.09.001

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title = "Human Cardiac Pericytes Are Susceptible to SARS-CoV-2 Infection",

abstract = "COVID-19 is associated with serious cardiovascular complications, with incompletely understood mechanism(s). Pericytes have key functions in supporting endothelial cells and maintaining vascular integrity. We demonstrate that human cardiac pericytes are permissive to SARS-CoV-2 infection in organotypic slice and primary cell cultures. Viral entry into pericytes is mediated by endosomal proteases, and infection leads to up-regulation of inflammatory markers, vasoactive mediators, and nuclear factor kappa-B–dependent cell death. Furthermore, we present evidence of cardiac pericyte infection in COVID-19 myocarditis patients. These data demonstrate that human cardiac pericytes are susceptible to SARS-CoV-2 infection and suggest a role for pericyte infection in COVID-19.",

keywords = "COVID-19, SARS-CoV-2, cardiovascular disease, pericytes",

author = "Brumback, {Brittany D.} and Oleksandr Dmytrenko and Robinson, {Ashley N.} and Bailey, {Adam L.} and Pan Ma and Jing Liu and Hicks, {Stephanie C.} and Sherwin Ng and Gang Li and Zhang, {David M.} and Lipovsky, {Catherine E.} and Lin, {Chieh Yu} and Diamond, {Michael S.} and Lavine, {Kory J.} and Rentschler, {Stacey L.}",

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year = "2023",

month = feb,

doi = "10.1016/j.jacbts.2022.09.001",

language = "English",

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AU - Brumback, Brittany D.

AU - Dmytrenko, Oleksandr

AU - Robinson, Ashley N.

AU - Bailey, Adam L.

AU - Ma, Pan

AU - Liu, Jing

AU - Hicks, Stephanie C.

AU - Ng, Sherwin

AU - Li, Gang

AU - Zhang, David M.

AU - Lipovsky, Catherine E.

AU - Lin, Chieh Yu

AU - Diamond, Michael S.

AU - Lavine, Kory J.

AU - Rentschler, Stacey L.

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AB - COVID-19 is associated with serious cardiovascular complications, with incompletely understood mechanism(s). Pericytes have key functions in supporting endothelial cells and maintaining vascular integrity. We demonstrate that human cardiac pericytes are permissive to SARS-CoV-2 infection in organotypic slice and primary cell cultures. Viral entry into pericytes is mediated by endosomal proteases, and infection leads to up-regulation of inflammatory markers, vasoactive mediators, and nuclear factor kappa-B–dependent cell death. Furthermore, we present evidence of cardiac pericyte infection in COVID-19 myocarditis patients. These data demonstrate that human cardiac pericytes are susceptible to SARS-CoV-2 infection and suggest a role for pericyte infection in COVID-19.

KW - COVID-19

KW - SARS-CoV-2

KW - cardiovascular disease

KW - pericytes

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U2 - 10.1016/j.jacbts.2022.09.001

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AN - SCOPUS:85139729528

SN - 2452-302X

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JO - JACC: Basic to Translational Science

JF - JACC: Basic to Translational Science

IS - 2

ER -

Human Cardiac Pericytes Are Susceptible to SARS-CoV-2 Infection

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