TY - JOUR
T1 - Human astrocytes and cytokines
T2 - Tumor necrosis factor alpha and interferon gamma do not promote astrocytic proliferation
AU - Moretto, G.
AU - Yoo, A.
AU - Kim, S. U.
PY - 1993/3/5
Y1 - 1993/3/5
N2 - Astrocyte cell cultures were established from human fetal brains and exposed to human recombinant tumor necrosis factor alpha (TNFα) and human recombinant interferon gamma (IFNγ), and the proliferative response was assessed by bromodeoxyuridine-immunostaining technique. TNFα showed an inhibitory activity, while IFNγ induced a slight increase in the number of astrocytes undergoing cell division. However, in both experiments differences were not statistically significant. Previous studies have demonstrated that TNFα and IFNγ are potent mitogens for adult astrocytes in both in vivo and in vitro conditions. Our results suggest that in developing brain the immunomodulatory activity of cytokines, TNFα and IFNγ, is different from that observed in adult brain.
AB - Astrocyte cell cultures were established from human fetal brains and exposed to human recombinant tumor necrosis factor alpha (TNFα) and human recombinant interferon gamma (IFNγ), and the proliferative response was assessed by bromodeoxyuridine-immunostaining technique. TNFα showed an inhibitory activity, while IFNγ induced a slight increase in the number of astrocytes undergoing cell division. However, in both experiments differences were not statistically significant. Previous studies have demonstrated that TNFα and IFNγ are potent mitogens for adult astrocytes in both in vivo and in vitro conditions. Our results suggest that in developing brain the immunomodulatory activity of cytokines, TNFα and IFNγ, is different from that observed in adult brain.
KW - Astrocyte
KW - Bromodeoxyuridine
KW - Cytokine
KW - Human
KW - Interferon gamma
KW - Tumor necrosis factor alpha
UR - http://www.scopus.com/inward/record.url?scp=0027167827&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(93)90034-I
DO - 10.1016/0304-3940(93)90034-I
M3 - Article
C2 - 8469430
AN - SCOPUS:0027167827
VL - 151
SP - 17
EP - 20
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -